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首页> 外文期刊>Cellular Physiology and Biochemistry >S100B Serves as a Ca ~(2+) sensor for ROS-GC1 guanylate cyclase in cones but not in rods of the murine retina
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S100B Serves as a Ca ~(2+) sensor for ROS-GC1 guanylate cyclase in cones but not in rods of the murine retina

机译：S100B在视锥细胞中充当ROS-GC1鸟苷酸环化酶的Ca〜（2+）传感器，但在鼠视网膜视杆中不起作用

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摘要

Rod outer segment membrane guanylate cyclase (ROS-GC1) is a bimodal Ca ~(2+) signal transduction switch. Lowering [Ca ~(2+)] i from 200 to 20 nM progressively turns it "ON" as does raising [Ca ~(2+)] _i from 500 to 5000 nM. The mode operating at lower [Ca ~(2+)] _i plays a vital role in phototransduction in both rods and cones. The physiological function of the mode operating at elevated [Ca ~(2+)] _i is not known. Through comprehensive studies on mice involving gene deletions, biochemistry, immunohistochemistry, electroretinograms and single cell recordings, the present study demonstrates that the Ca ~(2+)-sensor S100B coexists with and is physiologically linked to ROS-GC1 in cones but not in rods. It up-regulates ROS-GC1 activity with a K _(1/2) for Ca ~(2+) greater than 500 nM and modulates the transmission of neural signals to cone ON-bipolar cells. Furthermore, a possibility is raised that under pathological conditions where [Ca ~(2+)] i levels rise to and perhaps even enter the micromolar range, the S100B signaling switch will be turned "ON" causing an explosive production of CNG channel opening and further rise in [Ca ~(2+)] _i in cone outer segments. The findings define a new cone-specific Ca ~(2+)-dependent feature of photoreceptors and expand our understanding of the operational principles of phototransduction machinery.
机译：杆外段膜鸟苷酸环化酶（ROS-GC1）是一种双峰Ca〜（2+）信号转导开关。将[Ca〜（2+）] _i从200降低到20 nM，将[Ca〜（2+）] _i从500提升到5000 nM。 [Ca〜（2+）] _i较低的模式在棒和锥的光电导中都起着至关重要的作用。在[Ca〜（2+）] _i升高时工作的模式的生理功能尚不清楚。通过对小鼠的全面研究，涉及基因缺失，生物化学，免疫组织化学，视网膜电图和单细胞记录，本研究表明，Ca〜（2+）传感器S100B与ROS-GC1共存并在生理上与ROS-GC1共同存在，但在视锥细胞中不存在。 。它以大于500 nM的Ca〜（2+）的K _（1/2）上调ROS-GC1活性，并调节神经信号向视锥细胞双极型细胞的传递。此外，在[Ca〜（2+）] i水平升高甚至可能进入微摩尔范围的病理条件下，S100B信号开关将转为“ ON”，从而引起爆炸性的CNG通道打开和释放的可能性。圆锥外段的[Ca〜（2+）] _i进一步升高。这些发现定义了一种新的视锥细胞特定的Ca〜（2+）依赖性特征，并扩展了我们对光转导机械操作原理的理解。

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