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首页> 外文期刊>Radiation and Environmental Biophysics >Frozen human cells can record radiation damage accumulated during space flight: mutation induction and radioadaptation
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Frozen human cells can record radiation damage accumulated during space flight: mutation induction and radioadaptation

机译：冷冻的人类细胞可以记录太空飞行过程中积累的辐射损伤：突变诱导和放射适应

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To estimate the space-radiation effects separately from other space-environmental effects such as microgravity, frozen human lymphoblastoid TK6 cells were sent to the "Kibo" module of the International Space Station (ISS), preserved under frozen condition during the mission and finally recovered to Earth (after a total of 134 days flight, 72 mSv). Biological assays were performed on the cells recovered to Earth. We observed a tendency of increase (2.3-fold) in thymidine kinase deficient (TK~-) mutations over the ground control. Loss of heterozygosity (LOH) analysis on the mutants alsodemonstrated a tendency of increase in proportion of the large deletion (beyond the TK locus) events, 6/41 in the inflight samples and 1/17 in the ground control. Furthermore, in-flight samples exhibited 48% of the ground-control level in TK~- mutation frequency upon exposure to a subsequent 2 Gy dose of X-rays, suggesting a tendency of radioadaptation when compared with the ground-control samples. The tendency of radioadaptation was also supported by the post-flight assays on DNA double-strand break repair: a 1.8- and 1.7-fold higher efficiency of in-flight samples compared to ground control via non-homologous end-joining and homologous recombination, respectively. These observations suggest that this system can be used as a biodosimeter, because DNA damage generated by space radiation is considered to be accumulated in the cells preserved frozen during the mission, Furthermore, this system is also suggested to be applicable for evaluating various cellular responses to low-dose space radiation, providing a better understanding of biological space-radiation effects as well as estimation of health influences of future space explores.
机译：为了与其他空间环境影响（例如微重力）分开估计空间辐射效应，将冷冻的人类淋巴母细胞TK6细胞发送到国际空间站（ISS）的“ Kibo”模块，在任务期间在冷冻条件下保存并最终恢复到地球（总共飞行134天，飞行力为72毫西弗）。对回收到地球的细胞进行了生物测定。我们观察到与地面对照相比，胸苷激酶缺陷（TK〜-）突变增加（2.3倍）的趋势。突变体的杂合性丧失（LOH）分析也证明了大缺失（超出TK基因座）事件比例的增加趋势，机上样品为6/41，地面对照为1/17。此外，机上样品在暴露于随后的2 Gy剂量的X射线中时，其TK〜-突变频率显示出地面对照水平的48％，这表明与地面对照样品相比，存在放射适应的趋势。 DNA双链断裂修复的飞行后测定也支持了放射适应的趋势：与通过非同源末端连接和同源重组进行的地面对照相比，飞行中样品的效率高1.8到1.7倍，分别。这些观察结果表明，该系统可以用作生物剂量计，因为太空辐射产生的DNA损伤被认为是在任务期间冷冻保存的细胞中积累的。此外，该系统还建议可用于评估对低剂量空间辐射，可以更好地了解生物空间辐射的影响以及对未来空间探索的健康影响的估算。

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