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首页> 外文期刊>Radiation Physics and Chemistry >Effect of ionizing radiation on the quantitative detection of Salmonella using real-time PCR
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Effect of ionizing radiation on the quantitative detection of Salmonella using real-time PCR

机译：电离辐射对实时荧光定量PCR沙门氏菌定量检测的影响

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摘要

Food irradiation is an economically viable technology for inactivating foodborne pathogens,but irradiation can mask pathogens in unhygienically prepared food.The aim of this study was to investigate the effect of irradiation treatment on the detection of Salmonella using real-time PCR.Three commercially available kits were tested,of which the InstaGene Matrix procedure was most effective in preparing template DNA from Salmonella exposed to radiation in broth culture.The minimum level of detection by real-time PCR combined with InstaGene Matrix was 3 log units of Salmonella per milliliter.However,when pure cultures of Salmonella were irradiated at 3 and 5 kGy,the cycle threshold(C_T)increased 1-1.5-fold compared to irradiation at 0 and 1 kGy.This indicated that irradiation treatment may result in an underestimation of bacterial counts due to radiation-induced DNA lesions.We also compared C_T values in inoculated chicken homogenates before and after irradiation,which in this model caused a 1.3-3.3-fold underestimation of bacterial counts with respect to irradiation dose.
机译：食品辐照是灭活食源性病原体的一种经济可行的技术，但辐照可以掩盖未经卫生处理的食品中的病原体。本研究的目的是研究辐照处理对实时PCR检测沙门氏菌的影响。三种市售试剂盒进行了测试，其中InstaGene Matrix方法最有效地从肉汤中暴露于辐射的沙门氏菌中制备模板DNA。实时PCR与InstaGene Matrix结合检测的最低水平是每毫升3个log单位沙门氏菌。当分别以3和5 kGy辐照沙门氏菌培养物时，循环阈值（C_T）比以0和1 kGy辐照要高1-1.5倍。这表明辐照处理可能会由于辐照而导致细菌计数低估诱导的DNA损伤。我们还比较了接种鸡匀浆在照射前后的C_T值，该值在该模型中导致相对于辐射剂量，细菌计数低估了1.3-3.3倍。

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