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首页> 外文期刊>Respirology : >Low-level mechanical strain induces extracellular signal-regulated kinase 1/2 activation in alveolar epithelial cells.
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Low-level mechanical strain induces extracellular signal-regulated kinase 1/2 activation in alveolar epithelial cells.

机译：低水平的机械应变在肺泡上皮细胞中诱导细胞外信号调节激酶1/2活化。

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BACKGROUND AND OBJECTIVE: The pattern and the degree of mechanical stimuli may determine cellular responses, but little is known about how low magnitude stimuli are converted into biochemical signals in alveolar epithelial cells (AEC). The aim of this study was to explore whether extracellular signal-regulated kinases 1/2 (ERK1/2) are activated by low-level strain in A549 cells and how mechanical factors affect ERK1/2 phosphorylation. METHODS: A549 cells (an AEC line) were exposed to cyclic tensile strain via a four-point bending system, with strains of different magnitude (437, 874, 1748, 3496 microstrain), duration (5, 15, 30, 60, 120 min) and frequency (0.5, 1 Hz). Phosphorylation of ERK1/2 proteins was assessed by western blot. RESULTS: Maximal ERK1/2 phosphorylation occurred in the 874 microstrain group (a 2.25-fold increase, P < 0.01). In this group, the peak response occurred after 30 min of exposure and slowly decreased to baseline after 90 min. Static strain did not produce a statistically significant increase in ERK1/2 phosphorylation, whereas a frequency of 0.5 Hz produced a 4.56-fold increase compared with the control (P < 0.05). A 10.87-fold increase in response with frequency of 1 Hz was found. CONCLUSION: Low-level strain activates ERK1/2 in A549 cells. ERK1/2 may be the key signalling molecules mediating strain-induced cellular responses.
机译：背景与目的：机械刺激的方式和程度可能决定细胞的反应，但是关于低强度刺激在肺泡上皮细胞（AEC）中如何转化为生化信号的知之甚少。这项研究的目的是探讨低水平菌株在A549细胞中是否激活了细胞外信号调节激酶1/2（ERK1 / 2），以及机械因素如何影响ERK1 / 2磷酸化。方法：通过四点弯曲系统将A549细胞（一条AEC线）暴露于周期性拉伸应变，其应变大小不同（437、874、1748、3496微应变），持续时间（5、15、30、60、120 min）和频率（0.5，1 Hz）。通过蛋白质印迹评估ERK1 / 2蛋白的磷酸化。结果：874微应变组中最大的ERK1 / 2磷酸化发生（增加2.25倍，P <0.01）。在该组中，在暴露30分钟后出现峰值响应，在90分钟后缓慢下降至基线。静态应变未产生ERK1 / 2磷酸化的统计学显着增加，而与对照相比，0.5 Hz的频率产生了4.56倍的增加（P <0.05）。发现以1 Hz的频率响应增加了10.87倍。结论：低水平的菌株激活了A549细胞中的ERK1 / 2。 ERK1 / 2可能是介导应变诱导细胞反应的关键信号分子。

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