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首页> 外文期刊>Respirology : >In vitro cytotoxicity of CD8+ T cells in multi-drug-resistant tuberculosis. A preliminary report.
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In vitro cytotoxicity of CD8+ T cells in multi-drug-resistant tuberculosis. A preliminary report.

机译：CD8 + T细胞在多重耐药结核中的体外细胞毒性。初步报告。

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BACKGROUND AND OBJECTIVE: Specific CD8+ T-cell cytotoxicity has been recognized as being involved in the elimination of drug-susceptible tuberculosis (DS-TB). Given that there is currently no information on the cytotoxic effector functions of CD8+ T cells in multi-drug-resistant tuberculosis (MDR-TB), our objective was to analyse the cytotoxic activity, both basal and stimulated, of CD8+ T cells from MDR-TB patients and compare it with that of DS-TB patients, as well as purified protein derivative (PPD)+ and PPD- subjects. METHODS: Cytotoxic activity of CD8+ T cells from MDR-TB patients, DS-TB patients, PPD+ and PPD- subjects was measured by a colorimetric assay, using H37Rv culture filtrate protein as the antigenic stimulus. RESULTS: Twenty-eight subjects were studied (7 MDR-TB patients, 7 DS-TB patients, 7 PPD+ subjects and 7 PPD- subjects). In the presence of the antigenic stimulus, the cytotoxic activity of CD8+ T cells from MDR-TB patients (% lysis) increased from 6.7% to 59.6% (P < 0.001). In DS-TB patients lysis increased from 3.2% to 22.5% (P < 0.001), whereas in PPD+ subjects it increased from 2.7% to 12.0% (P < 0.001) and in PPD- subjects from 1.3% to 3.2% (P < 0.001). Basal cytotoxic activity was significantly higher for MDR-TB patients than PPD+ and PPD- subjects (P = 0.003), but not compared with that for DS-TB patients (P = 0.05). Stimulated cytotoxic activity was highest for MDR-TB patients. CONCLUSIONS: CD8+ T cells from MDR-TB patients showed an exaggerated cytotoxic activity after antigenic stimulation. Further studies are required to elucidate the role of this response in the immunopathogenesis of MDR-TB.
机译：背景与目的：特定的CD8 + T细胞细胞毒性已被认为与消除药物敏感性结核（DS-TB）有关。鉴于目前尚无关于耐多药结核病（MDR-TB）中CD8 + T细胞的细胞毒效应子功能的信息，我们的目标是分析MDR-CD8 + CD8 + T细胞的基础和受刺激细胞毒活性。结核病患者，并将其与DS-TB患者以及纯化的蛋白衍生物（PPD）+和PPD-患者进行比较。方法：采用H37Rv培养滤液蛋白作为抗原刺激物，通过比色法测定了MDR-TB患者，DS-TB患者，PPD +和PPD-受试者的CD8 + T细胞的细胞毒活性。结果：共研究了28名受试者（7名耐多药结核病患者，7名DS-TB患者，7名PPD +受试者和7名PPD-受试者）。在存在抗原刺激的情况下，MDR-TB患者的CD8 + T细胞的细胞毒性活性（裂解百分率）从6.7％增加到59.6％（P <0.001）。在DS-TB患者中，溶解度从3.2％增加到22.5％（P <0.001），而在PPD +患者中，溶解度从2.7％增加到12.0％（P <0.001），在PPD-患者中，溶解度从1.3％增加到3.2％（P < 0.001）。 MDR-TB患者的基础细胞毒活性明显高于PPD +和PPD-受试者（P = 0.003），但与DS-TB患者相比无统计学意义（P = 0.05）。耐多药结核病患者的刺激细胞毒性活性最高。结论：MDR-TB患者的CD8 + T细胞在抗原刺激后表现出夸大的细胞毒活性。需要进一步的研究来阐明这种反应在耐多药结核病的免疫发病机理中的作用。

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