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首页> 外文期刊>Rhinology >Nasal RANTES and eotaxin production pattern in response to rhinovirus infection.
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Nasal RANTES and eotaxin production pattern in response to rhinovirus infection.

机译：对鼻病毒感染的鼻腔RANTES和嗜酸性粒细胞趋化因子生产模式。

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Tissue eosinophilia is a hallmark of nasal polyposis and its pathogenesis is an area of high interest. RANTES and eotaxin are both known to recruit eosinophils, however, the mechanisms triggering their induction are still uncertain, and viral infections have been suggested to be involved in this process. Therefore, we investigated whether rhinovirus infection is a stimulus for RANTES and eotaxin expression and production. Fibroblasts were cultured from healthy nasal mucosa obtained during endonasal surgery. Cultured cells were infected with human rhinovirus-16 for one to 72 hours. Following total RNA isolation and reverse transcription, RANTES- and eotaxin-mRNA levels were analyzed. In addition, RANTES and eotaxin secretion was measured in culture supernatants by means of an ELISA. Rhinovirus infection induces RANTES-mRNA expression as early as one hour after infection, persisting for up to 72 hours. Eotaxin-mRNA profiles did not alter significantly from control. Protein production was confirmatory forboth chemokines, indicating distinct translational latency. Our data suggest that RANTES functions as a host defence mechanism responding to rhinovirus infection, thus supporting a linkage between rhinovirus infections and the pathogenesis of nasal polyposis.
机译：组织嗜酸性粒细胞增多症是鼻息肉病的标志，其发病机制是人们高度关注的领域。 RANTES和嗜酸性粒细胞趋化因子都可以募集嗜酸性粒细胞，但是，诱导其诱导的机制仍然不确定，并且已表明病毒感染与该过程有关。因此，我们调查了鼻病毒感染是否对RANTES和嗜酸性粒细胞趋化因子的表达和产生有刺激作用。从在鼻内手术期间获得的健康的鼻粘膜培养成纤维细胞。用人鼻病毒-16感染培养的细胞1-72小时。在总RNA分离和逆转录后，分析了RANTES和eotaxin-mRNA水平。另外，通过ELISA测量培养上清液中的RANTES和嗜酸性粒细胞趋化因子的分泌。鼻病毒感染最早在感染后一小时诱导RANTES-mRNA表达，持续长达72小时。嗜酸性粒细胞趋化因子-mRNA配置文件与对照组相比没有明显改变。两种趋化因子的蛋白产生都是确定的，表明明显的翻译潜伏期。我们的数据表明，RANTES是响应鼻病毒感染的宿主防御机制，从而支持了鼻病毒感染与鼻息肉病发病机制之间的联系。

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