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Effects of tetrabutylhydroperoxide on hepatocyte ion channels.

机译:氢过氧化四丁基对肝细胞离子通道的影响。

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摘要

As shown previously, tetrabutylhydroperoxide (t-BOOH) shrinks hepatocytes by release of cellular K(+). The hyperpolarization, the increase of K(+) selectivity, and the Ba(2+) sensitivity of K(+) release have been taken as evidence of activation of K(+) channels. To further define the ion channels involved, patch-clamp studies have been performed on rat hepatocytes. The data show that 0.1 mmol/l t-BOOH inhibits an inwardly rectifying K(+) channel and simultaneously activates a 35-pS K(+) channel. It is suggested that the activation of the latter channel population contributes to the observed K(+) release of hepatocytes following exposure to t-BOOH.
机译:如前所述,四丁基氢过氧化物(t-BOOH)通过释放细胞K(+)来收缩肝细胞。超极化,K(+)选择性的增加和K(+)释放的Ba(2+)敏感性已被视为激活K(+)通道的证据。为了进一步确定所涉及的离子通道,已经在大鼠肝细胞上进行了膜片钳研究。数据显示,0.1 mmol / l的t-BOOH抑制向内整流的K(+)通道,同时激活35-pS K(+)通道。建议后一个通道群体的激活有助于观察到暴露于t-BOOH后肝细胞的K(+)释放。

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