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首页> 外文期刊>Cellular Physiology and Biochemistry >Minor role of Cl- secretion in non-cystic fibrosis and cystic fibrosis human nasal epithelium.
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Minor role of Cl- secretion in non-cystic fibrosis and cystic fibrosis human nasal epithelium.

机译：Cl-分泌在非囊性纤维化和人鼻上皮囊性纤维化中的次要作用。

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Na+ and Cl- currents were studied in primary cultures of human nasal epithelium derived from non-cystic fibrosis (non-CF) and cystic fibrosis (CF) patients. We found that Na+ absorption dominates transepithelial transport and the Na+ current contains an amiloride-sensitive and amiloride-insensitive component. In non-CF tissue both components contribute about equally to the entire short-circuit current (ISC), whereas in CF tissues the major part of the current is amiloride-sensitive. Na+ removal reduced ISC to values close to zero. Several Cl- channel blockers were used to identify the remaining tiny Na+-independent current. Under unstimulated, physiological conditions in the presence of Cl- on both sides and amiloride on the apical side of the epithelium diphenylamine-2-carboxic acid (DPC), 4,4'-diisothiocyanatostilbene-2, 2'- disulfonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) failed to induce clearcut inhibition of ISC. cAMP as well as ATP did not affect ISC either in CF or in non-CF epithelia. Reduction of apical Cl- increased ISC and depolarized transepithelial potential; however, the observed increase was insensitive to DIDS, DPC and NPPB. From these data we conclude that Cl- conductances in primary cultures of human nasal epithelium derived from CF patients as well as from non-CF patients are present only in low numbers or do not contribute significantly to transepithelial ion transport.
机译：在来自非囊性纤维化（non-CF）和囊性纤维化（CF）患者的人鼻上皮细胞原代培养物中研究了Na +和Cl-电流。我们发现Na +吸收主导跨上皮运输，并且Na +电流包含对阿米洛利敏感和对阿米洛利不敏感的成分。在非CF组织中，两种成分对整个短路电流（ISC）的贡献大致相等，而在CF组织中，电流的主要部分对阿米洛利敏感。 Na +的去除将ISC降低到接近零的值。几种Cl通道阻滞剂用于鉴定剩余的微小Na +独立电流。在无刺激的生理条件下，上皮二苯胺-2-羧酸（DPC），4,4'-二异硫氰酸根合芪2、2'-二磺酸（DIDS）的两侧均存在Cl-，顶侧存在阿米洛利和5-硝基-2-（3-苯基丙基氨基）-苯甲酸（NPPB）不能诱导ISC的明确抑制。 cAMP和ATP在CF或非CF上皮中均不影响ISC。减少根尖Cl-增加的ISC和去极化的上皮电位;然而，观察到的增加对DIDS，DPC和NPPB不敏感。根据这些数据，我们得出结论，源自CF患者和非CF患者的人鼻上皮原代培养物中的Cl-传导仅少量存在，或对跨上皮离子转运没有显着贡献。

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