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首页> 外文期刊>Rheumatology >Expression of B-cell activating factor of the tumour necrosis factor family (BAFF) in T cells in active systemic lupus erythematosus: the role of BAFF in T cell-dependent B cell pathogenic autoantibody production.
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Expression of B-cell activating factor of the tumour necrosis factor family (BAFF) in T cells in active systemic lupus erythematosus: the role of BAFF in T cell-dependent B cell pathogenic autoantibody production.

机译：活动性系统性红斑狼疮中T细胞中肿瘤坏死因子家族（BAFF）B细胞活化因子的表达：BAFF在依赖T细胞的B细胞致病性自身抗体产生中的作用。

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摘要

OBJECTIVES: To determine whether B cell activating factor of the tumour necrosis factor family (BAFF) is involved in T cell-dependent B cell pathogenic autoantibody production in systemic lupus erythematosus (SLE). METHODS: Peripheral blood mononuclear cells (PBMCs) from 23 SLE patients were analysed by flow cytometry to examine the intracellular expression of BAFF in CD4+ and CD8+ T cells and the surface expression of BAFF-receptor (R) and TACI on CD20+ B cells. Moreover, peripheral blood was used to determine the level of BAFF messenger RNA (mRNA) in CD4+ and CD8+ T cells and the level of BAFF-R mRNA in CD20+ B cells. Blocking of BAFF function with TACI-Ig measured anti-double-stranded DNA (dsDNA) antibodies by enzyme-linked immunosorbent assay (ELISA). RESULTS: CD4+ and CD8+ T cells from patients with active SLE expressed intracellular BAFF whereas those from normal subjects did not. BAFF-R and TACI were expressed on B cells from both normal controls and patients with active SLE and there was no significant difference. CD4+ and CD8+ T cells from SLE patients expressed BAFF mRNA whereas those from normal controls did not. Expression of BAFF-R mRNA in CD20+ B cells showed no significant difference between SLE patients and normal controls. TACI-Ig suppressed spontaneous in vitro T cell-dependent B cell anti-dsDNA antibodies production on active SLE with kidney involvement. CONCLUSIONS: BAFF may play a pathogenic role in SLE by stimulating T cell-dependent B cell autoantibodies production. Blockade of BAFF is a promising therapeutic approach for SLE especially in patients with kidney involvement.
机译：目的：确定系统性红斑狼疮（SLE）中肿瘤坏死因子家族（BAFF）的B细胞活化因子是否参与T细胞依赖性B细胞致病性自身抗体的产生。方法：采用流式细胞术分析23例SLE患者的外周血单个核细胞（PBMC），以检测CD4 +和CD8 + T细胞中BAFF的细胞内表达以及CD20 + B细胞中BAFF-受体（R）和TACI的表面表达。此外，外周血用于确定CD4 +和CD8 + T细胞中BAFF信使RNA（mRNA）的水平以及CD20 + B细胞中BAFF-R mRNA的水平。通过酶联免疫吸附测定（ELISA），用TACI-Ig阻断BAFF功能可检测抗双链DNA（dsDNA）抗体。结果：活动性SLE患者的CD4 +和CD8 + T细胞表达细胞内BAFF，而正常受试者则不。正常对照组和活动性SLE患者的B细胞上均表达BAFF-R和TACI，差异无统计学意义。 SLE患者的CD4 +和CD8 + T细胞表达BAFF mRNA，而正常对照组则不表达。在SLE患者和正常对照之间，CD20 + B细胞中BAFF-R mRNA的表达没有明显差异。 TACI-Ig抑制活动性SLE伴肾脏累及时自发的体外T细胞依赖性B细胞抗dsDNA抗体产生。结论：BAFF可能通过刺激依赖T细胞的B细胞自身抗体的产生而在SLE中发挥致病作用。阻断BAFF是一种有前途的治疗SLE的方法，特别是在肾脏受累的患者中。

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