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首页> 外文期刊>RSC Advances >Physical and biological activities of newly designed, macro-pore-structure-controlled 3D fibrous poly(epsilon-caprolactone)/hydroxyapatite composite scaffolds
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Physical and biological activities of newly designed, macro-pore-structure-controlled 3D fibrous poly(epsilon-caprolactone)/hydroxyapatite composite scaffolds

机译：新型设计，大孔结构控制的3D纤维聚（ε-己内酯）/羟基磷灰石复合支架的物理和生物学活性

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摘要

Electrospun poly(epsilon-caprolactone) microanofibers have been used widely for tissue regeneration. However, their low three-dimensional (3D) shape-ability, difficult macro-pore-structure controllability, and relatively low bioactivities have been major obstacles to their use as various tissue regenerative scaffolds. In this study, we present a new 3D fibrous scaffold in which the macrosized pore structure was manipulated using an electrohydrodynamic jet process supplemented with in vitro mineralization to obtain a hydroxyapatite (HA) layer in simulated body fluid (SBF). The fabricated scaffolds were a mesh-like structure (average diameter of fiber bundles: 342 +/- 36 mu m; average diameter of microfiber: 8.5 +/- 1.2 mu m) with high porosity (>91%), macro-pore size (368 +/- 16 mu m), and 100% interconnected pores. The HA particles on the surface of the scaffolds were well developed after a 7 day incubation in SBF, and the 3D fibrous scaffolds showed markedly higher and more homogenous HA deposition compared with that of control scaffolds fabricated with solid free-form fabrication, due to the larger surface area of the fibrous bundles. The mineralized scaffolds were analyzed in terms of various physical and biological properties (water and protein absorption, mechanical properties, cell viability, DAPI/phalloidin staining, and osteogenic gene expression). Protein and water absorption of the fibrous scaffolds at 12 h were similar to 2.5-fold and similar to 2.3-fold greater than those of the control scaffolds. Additionally, proliferation of viable cells (MC3T3-E1 pre-osteoblasts) on the 3D fibrous scaffold improved significantly compared to the control. The osteogenic gene expression (alkaline phosphatase activity and calcium mineralization) levels of the fibrous scaffolds were significantly enhanced compared with those of the control scaffolds.
机译：电纺聚（ε-己内酯）微纤维/纳米纤维已被广泛用于组织再生。然而，它们低的三维（3D）成形能力，困难的大孔结构可控性以及相对较低的生物活性已成为其用作各种组织再生支架的主要障碍。在这项研究中，我们提出了一种新的3D纤维支架，其中使用电液动力射流工艺处理了大孔结构，并辅以了体外矿化作用，从而在模拟体液（SBF）中获得了羟基磷灰石（HA）层。制成的支架是网状结构（纤维束的平均直径：342 +/- 36微米;微纤维的平均直径：8.5 +/- 1.2微米），具有高孔隙率（> 91％），大孔径（368 +/- 16微米）和100％相互连通的孔。在SBF中孵育7天后，支架表面上的HA颗粒发育良好，并且3D纤维支架与采用固体自由形式制造的对照支架相比，显示出更高，更均匀的HA沉积。纤维束的表面积较大。根据各种物理和生物学特性（水和蛋白质吸收，机械特性，细胞生存力，DAPI / phalloidin染色和成骨基因表达）对矿化的支架进行了分析。纤维支架在12 h的蛋白质和吸水率比对照支架高2.5倍和2.3倍。另外，与对照相比，活细胞（MC3T3-E1成骨细胞）在3D纤维支架上的增殖显着改善。与对照支架相比，纤维支架的成骨基因表达（碱性磷酸酶活性和钙矿化）水平显着提高。

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