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Hydration-sensitive gene expression in brain

机译：水合敏感性基因在大脑中的表达

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摘要

Dehydration has a profound influence on neuroexcitability. The mechanisms remained, however, incompletely understood. The present study addressed the effect of water deprivation on gene expression in the brain. To this end, animals were exposed to a 24 hours deprivation of drinking water and neuronal gene expression was determined by microarray technology with subsequent confirmation by RT-PCR. As a result, water deprivation was followed by significant upregulation of clathrin (light polypeptide Lcb), serum/glucocorticoid-regulated kinase (SGK) 1, and protein kinase A (PRKA) anchor protein 8-like. Water deprivation led to downregulation of janus kinase and microtubule interacting protein 1, neuronal PAS domain protein 4, thrombomodulin, purinergic receptor P2Y - G-protein coupled 13 gene, gap junction protein beta 1, neurotrophin 3, hyaluronan and proteoglycan link protein 1, G protein-coupled receptor 19, CD93 antigen, forkhead box P1, suppressor of cytokine signaling 3, apelin, immunity-related GTPase family M, serine (or cysteine) peptidase inhibitor clade B member 1a, serine (or cysteine) peptidase inhibitor clade H member 1, glutathion peroxidase 8 (putative), discs large (Drosophila) homolog-associated protein 1, zinc finger and BTB domain containing 3, and H2A histone family member V. Western blotting revealed the downregulation of forkhead box P1, serine (or cysteine) peptidase inhibitor clade H member 1, and gap junction protein beta 1 protein abundance paralleling the respective alterations of transcript levels. In conclusion, water deprivation influences the transcription of a wide variety of genes in the brain, which may participate in the orchestration of brain responses to water deprivation.
机译：脱水对神经兴奋性有深远的影响。但是，对这些机制的理解仍不完全。本研究解决了缺水对大脑中基因表达的影响。为此，使动物暴露于缺乏饮用水的24小时，并通过微阵列技术确定神经元基因表达，随后通过RT-PCR确认。结果，在缺水之后，网格蛋白（轻型多肽Lcb），血清/糖皮质激素调节的激酶（SGK）1和蛋白激酶A（PRKA）锚定蛋白8-like明显上调。缺水导致下颌静脉激酶和微管相互作用蛋白1，神经元PAS结构域蛋白4，血栓调节蛋白，嘌呤能受体P2Y-G蛋白偶联的13基因，间隙连接蛋白β1，神经营养蛋白3，透明质酸和蛋白聚糖连接蛋白1，G的下调。蛋白偶联受体19，CD93抗原，叉头盒P1，细胞因子信号转导抑制剂3，apelin，与免疫相关的GTPase家族M，丝氨酸（或半胱氨酸）肽酶抑制剂进化枝B成员1a，丝氨酸（或半胱氨酸）肽酶抑制剂进化枝H成员1，谷胱甘肽过氧化物酶8（假定），圆盘大（果蝇）同源相关蛋白1，含锌指和BTB结构域3，以及H2A组蛋白家族成员V.蛋白质印迹显示叉头盒P1，丝氨酸（或半胱氨酸）的下调。肽酶抑制剂进化枝H成员1和缺口连接蛋白β1蛋白的丰度与转录水平的相应变化平行。总之，缺水会影响大脑中多种基因的转录，这些基因可能参与协调大脑对缺水的反应。

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