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Aptasensor for label-free square-wave voltammetry detection of potassium ions based on gold nanoparticle amplification

机译：基于金纳米粒子扩增的用于无标记方波伏安法检测钾离子的Aptasensor

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Herein, we present a very simple and label-free square-wave voltammetry (SWV) aptasensor to detect potassium ions (K+), in which a K+-specific aptamer was used as an ion recognition element, and a redox couple [Fe(CN)(6)](3-/4-) as a redox probe. At the bare gold electrode, the redox couple [Fe(CN)(6)](3-/4-) can very easily access the electrode surface to produce a very strong SWV signal. At the K+-specific aptamer/gold electrode surface, in the presence of K+, the single-stranded DNA with guanine (G)-rich sequences could fold into a secondary structure, G-quadruplex, which resulted in less availability for a redox reaction, and led to a smaller SWV current. To improve signal intensity, gold nanoparticles (AuNPs) were anchored to a gold electrode surface which had been previously modified with self-assembled monolayers of p-aminothiophenol. The SWV peak current decreased with K+ concentration, and the plot of the SWV peak current against the logarithm of K+ concentration is linear over the range from 10 pM to 0.1 mu M and 0.5 mu M to 1 mM, with a detection limit of 0.13 pM. The aptasensor also showed a very good selectivity for K+ without being affected by the coexistence of other ions.
机译：在这里，我们提出了一种非常简单且无标记的方波伏安（SWV）适体传感器，用于检测钾离子（K +），其中使用K +特异性适体作为离子识别元件，并使用氧化还原对[Fe（CN ）（6）]（3- / 4-）作为氧化还原探针。在裸金电极上，氧化还原对[Fe（CN）（6）]（3- / 4-）可以很容易地进入电极表面，以产生非常强的SWV信号。在K +特异性适体/金电极表面上，在K +存在下，具有鸟嘌呤（G）富集序列的单链DNA可以折叠成二级结构G-quadruplex，导致氧化还原反应的可用性降低，并导致较小的SWV电流。为了提高信号强度，将金纳米颗粒（AuNPs）固定在预先用对氨基硫酚自组装单层修饰的金电极表面上。 SWV峰值电流随K +浓度而降低，并且SWV峰值电流相对于K +浓度的对数的曲线在10 pM至0.1μM和0.5μM至1 mM的范围内呈线性，检测极限为0.13 pM 。适体传感器还显示出对K +的非常好的选择性，而不受其他离子共存的影响。

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