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首页> 外文期刊>RSC Advances >Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells
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Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells

机译：桂皮提取物抑制幽门螺杆菌的生长和人胃上皮细胞中感染后白细胞介素8的表达

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摘要

Essential oils from leaves of Taiwan's indigenous cinnamon, Cinnamomum osmophloeum extracts (CEs), contain similar constituents to those of commercial cinnamons from dried barks of other Cinnamomum species. However, cinnamon bark extracts showed controversial in vitro and in vivo results in their antibacterial activities against H. pylori. Whether CEs inhibit H. pylori growth and inflammation of H. pylori-infected gastric epithelium is unknown. In this study, we used 4 h water distillation to obtain CEs, which comprised >95% trans-cinnamaldehyde and other 4 minor compounds that were confirmed by GC-MS and contained 1305.5 mu g ml(-1) of trans-cinnamaldehyde that was quantified by HPLC analysis. The minimal inhibitory concentration of trans-cinnamaldehyde in CEs against H. pylori was 40.8 mu g ml(-1) in 48 h co-cultures. We subsequently performed 2 cinnamon extract assays: 3 h treatment of CEs containing 6.3 mu g ml(-1) and 63 mu g ml(-1) of trans-cinnamaldehyde significantly inhibited IL-8 mRNA and protein expression in the 1 hour H. pylori-infected cells, whereas 6 h treatment of CEs containing 21 mu g ml(-1) of trans-cinnamaldehyde, but not CEs containing 10.5 mu g ml(-1) of trans-cinnamaldehyde, substantially downregulated IL-8 mRNA expression and these two CEs strongly suppressed IL-8 protein secretion in the 2 h H. pylori-infected cells. In the 2 h IL-1 beta-treated cells, both CEs and t-SSs containing 21 mu g ml(-1) and 10.5 mu g ml(-1) of trans-cinnamaldehyde for 6 h markedly downregulated IL-8 mRNA expression; CEs inhibited IL-8 protein production more potently than t-SSs when both contained the same concentration of trans-cinnamaldehyde at 21 mu g ml(-1). In conclusion, CEs concentration-dependently inhibit H. pylori growth and postinfectiously inhibit IL-8 mRNA and protein expression in H. pylori-and IL-1 beta-pretreated AGS cells. Moreover, CEs exhibited a stronger anti-inflammatory effect than trans-cinnamaldehyde, indicating that CEs can potentially be used to treat H. pylori infection.
机译：来自台湾本土肉桂叶的肉桂油提取物（CEs）的成分与其他肉桂干树皮的商业肉桂成分相似。但是，肉桂皮提取物在体外和体内显示出对幽门螺杆菌的抗菌活性，这引起了争议。 CEs是否抑制幽门螺杆菌的生长和感染幽门螺杆菌的胃上皮的炎症尚不清楚。在这项研究中，我们使用4 h水蒸馏获得CEs，其中包括> 95％的反式肉桂醛和其他4种次要化合物，这些化合物经GC-MS确认，并含有1305.5μg ml（-1）的反式肉桂醛，通过HPLC分析定量。在48 h共培养中，CE中反式肉桂醛对幽门螺杆菌的最小抑制浓度为40.8μg ml（-1）。我们随后进行了2次肉桂提取物检测：含有6.3μg ml（-1）和63μg ml（-1）反式肉桂醛的CE的3小时处理在1小时内显着抑制了IL-8 mRNA和蛋白质表达。幽门螺杆菌感染的细胞，而含有21μg ml（-1）反式肉桂醛的CE处理6 h，但不含含有10.5μg ml（-1）反式肉桂醛的CE处理，则实质上下调了IL-8 mRNA表达和这两个CE强烈抑制了幽门螺杆菌感染2 h细胞中IL-8蛋白的分泌。在经过2小时IL-1β处理的细胞中，CE和t-SS都含有21μg ml（-1）和10.5μg ml（-1）的反式肉桂醛，持续6 h时，IL-8 mRNA表达明显下调。 ;当CEs在21μg ml（-1）中包含相同浓度的反式肉桂醛时，它们比t-SSs更有效地抑制IL-8蛋白的产生。总之，CEs浓度依赖性抑制幽门螺杆菌的生长，并在感染后抑制幽门螺杆菌和IL-1β预处理的AGS细胞中IL-8 mRNA和蛋白表达。此外，CEs表现出比反式肉桂醛更强的抗炎作用，表明CEs可以潜在地用于治疗幽门螺杆菌感染。

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