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首页> 外文期刊>RSC Advances >In situ polymerization of aniline on carbon quantum dots: a new platform for ultrasensitive detection of glucose and hydrogen peroxide
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In situ polymerization of aniline on carbon quantum dots: a new platform for ultrasensitive detection of glucose and hydrogen peroxide

机译：苯胺在碳量子点上的原位聚合：超灵敏检测葡萄糖和过氧化氢的新平台

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摘要

A simple and ultrasensitive platform for the detection of glucose and hydrogen peroxide based on fluorescence resonance energy transfer between carbon quantum dots (CQDs) and polyaniline is described in this report. Briefly, during enzymatic oxidization of glucose, hydrogen peroxide is generated. The generated hydrogen peroxide, in turn, initiates oxidative polymerization of aniline on the surface of the CQDs in the presence of horseradish peroxidase. And the formation of a thin polyaniline layer efficiently quenches the fluorescence of the CQDs. It was observed that the quenching of the fluorescence is directly associated with the concentration of glucose and ultrasensitive detection of glucose down to submicromolar levels was achieved. In addition, since the formation of polyaniline on the CQD surface is directly associated with hydrogen peroxide, it was found that traces of hydrogen peroxide can be detected in a range of 0.5-50 mu M with good selectivity.
机译：本报告介绍了一种基于碳量子点（CQD）与聚苯胺之间的荧光共振能量转移来检测葡萄糖和过氧化氢的简单且超灵敏的平台。简而言之，在葡萄糖的酶促氧化过程中，产生了过氧化氢。在辣根过氧化物酶的存在下，生成的过氧化氢又会引发CQD表面苯胺的氧化聚合。聚苯胺薄层的形成有效地淬灭了CQD的荧光。观察到荧光的猝灭与葡萄糖的浓度直接相关，并且实现了低至亚微摩尔水平的葡萄糖的超灵敏检测。另外，由于在CQD表面上形成聚苯胺与过氧化氢直接相关，因此发现可以在0.5-50μM的范围内以良好的选择性检测到痕量的过氧化氢。

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