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Antioxidative and antimicrobial activities of the extracts from the seed coat of Bambara groundnut (Voandzeia subterranea)

机译:班巴拉花生(Voandzeia subterranea)种皮提取物的抗氧化和抗菌活性

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Antioxidative and antimicrobial activities of extracts from Bambara groundnut seed coat, prepared using water, ethanol and acetone as the media at various temperatures (30, 60, 90 degrees C) were investigated. Extraction yields and phenolic contents of Bambara groundnut seed coat extracts (BGSEs) varied from 5.21 to 13.23% and from 169 to 569 mg GAE per g dry extract, respectively. BGSEs showed varying antioxidative activities including DPPH, ABTS, hydroxyl and superoxide anion radical scavenging activities, ferric reducing antioxidant power and metal chelating activity, depending on extraction conditions (p < 0.05). Antioxidative activities of BGSE prepared using ethanol at 60 degrees C (BGSE(E60)) were stable in a wide pH range and with heat treatment. BGSE(E60) exhibited antioxidative activities in the lecithin liposome system and steamed chicken mince as evidenced by the retarded formation of conjugated diene, peroxide value and thiobarbituric acid reactive substances (TBARS) (p < 0.05). Additionally, the BGSE(E60) (2.5-30 mg ml(-1)) possessed antimicrobial activities against bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus), yeast (Candida albicans) and mold (Aspergillus niger), in a dose-dependent manner. Scanning and transmission electron microscopic studies also revealed the damage of microbial cells after being treated with BGSE(E60). No cytotoxicity of BGSE(E60) against the primate cell line (Vero) was observed. Therefore, extraction yields, total phenolic contents and antioxidative activities of BGSEs were governed by the extracting solvents and temperatures used. Additionally, BGSEs could be used as natural antioxidants and antimicrobial agents without cytotoxicity.
机译:研究了在不同温度(30、60、90摄氏度)下,以水,乙醇和丙酮为介质制备的班巴拉花生花生种皮提取物的抗氧化和抗菌活性。每克干提取物的Bambara花生种皮提取物(BGSE)的提取得率和酚含量分别为5.21%至13.23%和169至569 mg GAE。 BGSE表现出不同的抗氧化活性,包括DPPH,ABTS,羟基和超氧化物阴离子自由基清除活性,三价铁还原抗氧化能力和金属螯合活性,具体取决于提取条件(p <0.05)。使用乙醇在60摄氏度下制备的BGSE(BGSE(E60))的抗氧化活性在很宽的pH范围和热处理条件下均稳定。 BGSE(E60)在卵磷脂脂质体系统中表现出抗氧化活性,并且在蒸鸡肉末中表现出共轭二烯,过氧化物值和硫代巴比妥酸反应性物质(TBARS)的形成受阻(p <0.05)。此外,BGSE(E60)(2.5-30 mg ml(-1))对细菌(金黄色葡萄球菌,大肠杆菌,蜡状芽孢杆菌),酵母菌(白色念珠菌)和霉菌(黑曲霉)具有抗菌活性,依赖方式。扫描和透射电镜研究也显示了用BGSE(E60)处理后微生物细胞的损伤。没有观察到BGSE(E60)对灵长类细胞系(Vero)的细胞毒性。因此,BGSE的提取得率,总酚含量和抗氧化活性受提取溶剂和所用温度的控制。此外,BGSE可用作天然抗氧化剂和抗菌剂,而无细胞毒性。

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