• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Development and characterization of a novel Swarna-based herbo-metallic colloidal nano-formulation - inhibitor of Streptococcus mutans quorum sensing
【24h】

Development and characterization of a novel Swarna-based herbo-metallic colloidal nano-formulation - inhibitor of Streptococcus mutans quorum sensing

机译：新型基于Swarna的疱疹金属胶体纳米制剂的开发与表征-变形链球菌群体感应抑制剂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Herbo-metallic preparations such as bhasmas (ash) are used traditionally in Indian and Chinese medicinal systems. In Ayurveda, Swarna (gold) nanoparticles are used as Swarna bhasma to treat several clinical manifestations. While Usnea longissima, a medicinal lichen, ethnobotanical is known for the treatment of tooth cleaning and infectious diseases. The study aims to develop a herbo-metallic colloidal nano-formulation containing Swarna nanoparticles and polyphenols rich U. longissima extract (ULE) and evaluate its anti-quorum sensing (QS) property against Streptococcus mutans, an idea that has never been explored before, with a view towards combating the emergence of antibiotic resistance often linked with QS-regulated virulence factors and biofilms. The synthesized Uh-Au@Nano-CF was confirmed by a peak at 550 nm in the UV-visible spectrum. The obtained XRD data confirmed the crystalline nature of nanoparticles of 28 nm size. TEM image revealed that all the particles were spherical with a narrowsize range of 5-23 nm. The FTIR result clearly showed that the ULE containing secondary OH as functional groups induces encapsulation of nanoparticles. HPTLC and HPLC fingerprinting of ULE confirmed the presence of polyphenols, including orcinol, arabitol, apigenin, and usnic acid. The data from the inhibition of violacein production in C. violaceum 12 472 revealed that the Uh-Au@Nano-CF at sub-lethal concentrations (5, 10 and 15%) show potent anti-QS activity. The treatment of Uh-Au@Nano-CF was found to inhibit the secretion of S. mutans virulence factors, including acid production, ATPase, enolase, lactate dehydrogenase, protease, total exopolysaccharide content, and glucosidase. The Uh-Au@Nano-CF in a concentration dependent manner showed anti-biofilm activity, inhibiting biofilm formation. Eventually, it was also documented that the Uh-Au@Nano-CF at 15% dilution enhanced the susceptibility of S. mutans to its conventional antibiotics. This study introduces not only a novel antimicrobial herbo-metallic colloidal nano-formulation, but also explores its new biomedical application, which targets QS-regulated virulence factors and biofilm of S. mutans rather than its viability.
机译：传统上在印度和中国的医药系统中使用草药金属制剂，例如bhasmas（灰烬）。在阿育吠陀，Swarna（金）纳米粒子被用作Swarna bhasma，以治疗多种临床表现。药用地衣松萝（Usnea longissima）是一种药用植物，而植物植物药因其清洁牙齿和感染性疾病而闻名。这项研究旨在开发一种含有Swarna纳米颗粒和富含多酚的U. longissima提取物（ULE）的草本金属胶体纳米制剂，并评估其对变形链球菌的抗群体感应（QS）性能，这一想法以前从未探索过，为了对抗经常与QS调节的毒力因子和生物膜相关的抗生素耐药性的出现。合成的Uh-Au @ Nano-CF通过紫外可见光谱中550 nm处的峰确认。所获得的XRD数据证实了28nm尺寸的纳米颗粒的晶体性质。 TEM图像显示所有颗粒均为球形，粒径范围为5-23 nm。 FTIR结果清楚地表明，含有仲羟基作为官能团的ULE可以诱导纳米颗粒的包封。 ULE的HPTLC和HPLC指纹图谱证实了多酚的存在，其中包括豆蔻酚，阿拉伯糖醇，芹菜素和松萝酸。来自紫罗兰杆菌12 472中紫罗兰素生产抑制的数据表明，亚致死浓度（5％，10％和15％）的Uh-Au @ Nano-CF显示出有效的抗QS活性。发现Uh-Au @ Nano-CF的处理抑制变形链球菌毒力因子的分泌，包括产酸，ATP酶，烯醇酶，乳酸脱氢酶，蛋白酶，总外多糖含量和葡糖苷酶。 Uh-Au @ Nano-CF以浓度依赖性方式显示出抗生物膜活性，抑制了生物膜的形成。最终，也有文献证明，稀释15％的Uh-Au @ Nano-CF增强了变形链球菌对其常规抗生素的敏感性。这项研究不仅介绍了一种新型的抗菌金属-金属胶体纳米制剂，而且还探讨了其新的生物医学应用，其针对变形链球菌的QS调节毒力因子和生物膜而不是其生存力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号