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Rapid endophytic bacterial detection by enzyme incorporated MALDI MS

机译:通过酶结合MALDI MS快速检测内生细菌

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In this paper, we have attempted the use of MALDI-MS for the detection of bacteria from complex real world samples such as the root nodules of plants. We have employed lysozyme as the enzyme biosensor to enrich the bacterial molecular signatures from endophytic bacteria during the MALDI-MS analysis. We used the root nodules of Arachis hypogaea, L. as an endophytic model system to prove the enrichment of the endophytic bacterial signals for the rapid and direct identification of the endophytic bacteria. We have been able to demonstrate the functionality of lysozyme as an enzyme biosensor during the mass spectrometric analysis of complex plant tissues such as the root nodules, leading to a culture-free detection of the endophytic bacteria. We also report the direct identification of the specific dominant endophytic isolate using MALDI-MS combined with Biotyper software analysis and validation.
机译:在本文中,我们尝试使用MALDI-MS从复杂的现实世界样本(例如植物的根瘤)中检测细菌。我们已使用溶菌酶作为酶生物传感器,以在MALDI-MS分析过程中丰富内生细菌的细菌分子标记。我们使用了花生的根瘤作为内生模型系统,以证明内生细菌信号的富集,可以快速直接鉴定内生细菌。我们已经能够在复杂植物组织(例如根瘤)的质谱分析过程中证明溶菌酶作为酶生物传感器的功能,从而导致无培养物检测内生细菌。我们还报告了使用MALDI-MS结合Biotyper软件分析和验证对特定优势内生分离物的直接鉴定。

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