• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Development and validation of an UPLC method for the quantification of ethambutol in rat plasma
【24h】

Development and validation of an UPLC method for the quantification of ethambutol in rat plasma

机译：开发和验证用于定量大鼠血浆乙胺丁醇的UPLC方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: The effective monitoring of antitubercular drugs, including ethambutol (EMB), is important for the control of multiple drug resistance. The development of fast, facile, and inexpensive methods for the detection of low concentrations of EMB, especially near its minimum inhibitory concentration, is also important. Results: An UPLC method for estimating EMB in rat plasma was developed and validated. The method uses a small injection volume (1 mu l) and elutes EMB at 3.18 min, with 0.070 mu g ml(-1) and 0.250 mu g ml(-1) as the limits of detection and quantification, respectively. The intra-and interday accuracy was 106.01-108.82% and 107.80-109.00%, respectively, with a precision of <5% in both cases. The mean extraction recovery of EMB from rat plasma was >97% at three concentration levels, establishing the consistency and reproducibility (n = 6) of the method. EMB was found to be stable in plasma under different storage and processing conditions. Conclusion: This paper presents a highly sensitive and specific isocratic UPLC method for the rapid quantization of EMB for use in pharmacokinetics and therapeutic drug monitoring.
机译：背景：有效监测包括乙胺丁醇（EMB）在内的抗结核药物，对于控制多重耐药性至关重要。开发快速，简便且廉价的方法来检测低浓度的EMB（尤其是在其最小抑制浓度附近）也很重要。结果：建立并验证了用于估计大鼠血浆中EMB的UPLC方法。该方法使用少量进样量（1μl）并在3.18分钟时洗脱EMB，分别以0.070μgml（-1）和0.250μgml（-1）作为检测和定量的极限。日内和日间准确度分别为106.01-108.82％和107.80-109.00％，两种情况下的精确度均小于5％。在三个浓度水平下，从大鼠血浆中EMB的平均提取回收率> 97％，建立了该方法的一致性和重现性（n = 6）。发现EMB在不同的储存和加工条件下在血浆中是稳定的。结论：本文提出了一种高度灵敏，特异的等度UPLC方法，用于EMB的快速定量，可用于药代动力学和治疗药物监测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号