首页> 外文期刊>RSC Advances >Complete degradation of dimethyl phthalate by biochemical cooperation of the Bacillus thuringiensis strain isolated from cotton field soil
【24h】

Complete degradation of dimethyl phthalate by biochemical cooperation of the Bacillus thuringiensis strain isolated from cotton field soil

机译:棉田土壤中苏云金芽孢杆菌菌株生化协同完全降解邻苯二甲酸二甲酯

获取原文
获取原文并翻译 | 示例
           

摘要

Dimethyl phthalate (DMP), a phthalate ester, is widely used in cosmetics, perfumes, and plasticizers. It has been classified as a suspected endocrine disruptor by many countries. The present study describes the biodegradation of DMP by a new aerobic bacterium, isolated from soil samples of a cotton field by an enrichment culture technique utilizing DMP as the sole source of carbon and energy. The isolate was identified as Bacillus thuringiensis based on the morphological and biochemical characteristics as well as gene sequence analysis. Bacillus thuringiensis grows best in a mineral salt medium of pH 7.0 at 30 degrees C incubation for 48 hours. The effects of temperature, inoculum size, substrate concentration and incubation time on DMP degradation were also studied. Bacillus thuringiensis is able to biodegrade 400 mg L-1 of DMP under aerobic conditions with 99% degradation potential. A combination of GC and GC-MS analysis revealed a complete DMP biodegradation pathway. The results indicate that Bacillus thuringiensis may prove a promising source for DMP bioremediation at a commercial scale.
机译:邻苯二甲酸二甲酯(DMP)是一种邻苯二甲酸酯,广泛用于化妆品,香水和增塑剂中。许多国家已将其归类为可疑内分泌干扰物。本研究描述了一种新的好氧细菌对DMP的生物降解,该细菌通过利用DMP作为唯一碳和能量来源的富集培养技术从棉田土壤样品中分离出来。根据形态和生化特征以及基因序列分析,该分离株被鉴定为苏云金芽孢杆菌。苏云金芽孢杆菌在pH 7.0的无机盐培养基中30摄氏度孵育48小时生长最佳。还研究了温度,接种量,底物浓度和孵育时间对DMP降解的影响。苏云金芽孢杆菌能够在有氧条件下生物降解400 mg L-1的DMP,降解潜力为99%。 GC和GC-MS分析的结合显示了完整的DMP生物降解途径。结果表明,苏云金芽孢杆菌可能被证明是商业规模的DMP生物修复的有希望的来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号