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Purification of phosvitin phosphopeptides using macro-mesoporous TiO2

机译:使用大中孔TiO2纯化磷脂酰肌醇磷酸肽

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摘要

Macro-mesostructured titanium dioxide (MMTD) was synthesized, characterized and applied as an adsorbent in the purification of phosvitin phosphopeptides (PPPs) from egg-yolk protein hydrolysates successfully. The synthesized material was analyzed using Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption-desorption analysis and scanning electron microscopy (SEM). The resultant MMTD exhibited a uniform macroporous structure with worm-like mesoporous walls, and the particle sizes were around 22 mu m with the facility to be separated from aqueous solution. In the purification process, the adsorption of PPPs onto MMTD fitted well with the Freundlich model and pseudo-second-order kinetics model, and the maximum adsorption capacity reached 36.47 mg g(-1) in 20 min when the crude polypeptide concentration was 16 mg mL(-1). The purity of the obtained PPPs was relatively high with a nitrogen/phosphorus molar ratio (N/P) of 5.4. Moreover, the reusability of MMTD was satisfying in the twenty repeated adsorption-desorption cycles. Hence, the purification of PPPs using MMTD was highly efficient and could be scaled-up for practical application.
机译:合成,表征了介孔结构大的二氧化钛(MMTD),并将其用作吸附剂,成功地从蛋黄蛋白水解物中纯化出了磷光蛋白磷酸肽(PPPs)。使用傅立叶变换红外光谱(FT-IR),氮吸附-解吸分析和扫描电子显微镜(SEM)对合成的材料进行了分析。所得的MMTD表现出具有蠕虫状中孔壁的均匀大孔结构,并且具有从水溶液中分离的装置的粒径为约22μm。在纯化过程中,PPPs在MMTD上的吸附与Freundlich模型和拟二级动力学模型吻合得很好,当粗多肽浓度为16 mg时,在20 min内最大吸附量达到36.47 mg g(-1)。毫升(-1)。所获得的PPP的纯度相对较高,氮/磷的摩尔比(N / P)为5.4。而且,在二十个重复的吸附-解吸循环中,MMTD的可重用性令人满意。因此,使用MMTD纯化PPP的效率很高,可以扩大规模以用于实际应用。

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