Quaternary structures of GroEL and naive-Hsp60 chaperonins in solution: a combined SAXS-MD study

Spinello A.; Ortore M. G.; Spinozzi F.; Ricci C.; Barone G.; Gammazza A. Marino; Piccionello A. Palumbo

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Quaternary structures of GroEL and naive-Hsp60 chaperonins in solution: a combined SAXS-MD study

机译：GroEL和朴素的Hsp60伴侣蛋白在溶液中的季结构：结合SAXS-MD研究

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The quaternary structures of bacterial GroEL and human naive-Hsp60 chaperonins in physiological conditions have been investigated by an innovative approach based on a combination of synchrotron Small Angle X-ray Scattering (SAXS) in-solution experiments and molecular dynamics (MD) simulations. Low-resolution SAXS experiments over large and highly symmetric oligomers are analyzed on the basis of the high-resolution structure of the asymmetric protein monomers, provided by MD. The results reveal remarkable differences between the solution and the crystallographic structure of GroEL and between the solution structures of GroEL and of its human homologue Hsp60.

机译：通过将同步加速器小角X射线散射（SAXS）溶液内实验与分子动力学（MD）模拟相结合的创新方法，研究了生理条件下细菌GroEL和人类朴素Hsp60伴侣蛋白的四级结构。在MD提供的不对称蛋白质单体的高分辨率结构的基础上，分析了在大型且高度对称的低聚物上的低分辨率SAXS实验。结果揭示了GroEL的溶液和晶体结构之间以及GroEL及其人类同源物Hsp60的溶液结构之间存在显着差异。

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《RSC Advances》 |2015年第62期|共9页
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Spinello A.; Ortore M. G.; Spinozzi F.; Ricci C.; Barone G.; Gammazza A. Marino; Piccionello A. Palumbo;
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1. Quaternary structures of GroEL and naive-Hsp60 chaperonins in solution: a combined SAXS-MD study [J] . Spinello A., Ortore M. G., Spinozzi F., RSC Advances . 2015,第62期

机译：GroEL和朴素的Hsp60伴侣蛋白在溶液中的季结构：结合SAXS-MD研究
2. A thermophilic mini-chaperonin contains a conserved polypeptide-bindingsurface: Combined crystallographic and NMR studies of the GroEL apicaldomain with implications for substrate interactions [J] . Hua QX, Dementieva IS, Walsh MA, Journal of Molecular Biology . 2001,第3期

机译：嗜热的小分子伴侣蛋白含有保守的多肽结合表面：GroEL顶端结构域的结晶学和NMR结合研究，对底物相互作用有影响
3. Structural changes of the Escherichia coli GroEL-GroES chaperonins upon complex formation in solution: a neutron small angle scattering study [J] . Renata Stegmann, Elena Manakova, Manfred RoBle Journal of Structural Biology . 1998,第1期

机译：溶液中复合物形成后大肠杆菌GroEL-GroES伴侣蛋白的结构变化：中子小角散射研究
4. First Chaperonin GroEL Cluster Immobilized on Gold Nanoparticles as Functional Materials [C] . Hao K. Shen, P.K. Hashim, Daiki Kashiwagi 日本化学会春季年会講演予稿集 . 2018

机译：第一伴侣素腹股沟簇固定在金纳米粒子上作为功能材料
5. Single molecule studies of the chaperonin GroEL [D] . Frank, Gabriel A. 2010

机译：伴侣蛋白GroEL的单分子研究
6. Crystal and solution structures of the oligonucleotide d(ATGCGCAT)2: a combined X-ray and NMR study. [O] . G R Clark, D G Brown, M R Sanderson, 1990

机译：寡核苷酸d（ATGCGCAT）2的晶体和溶液结构：结合X射线和NMR研究。
7. Quaternary structures of GroEL and naïve-Hsp60 chaperonins in solution: A combined SAXS-MD study [O] . Spinello, A, Ortore, M, Spinozzi, F, 2015

机译：GroEL和朴素的Hsp60伴侣蛋白在溶液中的季结构：结合SAXS-MD研究

Quaternary structures of GroEL and naive-Hsp60 chaperonins in solution: a combined SAXS-MD study

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