...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Rheumatology >Neutrophils produce interleukin-17B in rheumatoid synovial tissue
【24h】

Neutrophils produce interleukin-17B in rheumatoid synovial tissue

机译：中性粒细胞在类风湿滑膜组织中产生白介素-17B

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: T helper 17 (Th17) and mast cells produce IL-17A in RA and critically contribute to the pathogenesis of RA. However, the complete IL-17 cytokine profile in RA is unknown. The aim of the study was to systematically study the expression of IL-17 family cytokines in RA. Methods: The expression of all IL-17 cytokines in RA synovium and pannus as well as in the synovium of OA was determined using quantitative RT-PCR (qRT-PCR). IL-17A and IL-17B were immunostained. Peripheral blood neutrophils were analysed for IL-17B. The effect of IL-17B alone or in combination with TNF-α was tested in vitro on fibroblasts and endothelial cells. Results: In all tissues IL-17B was the most expressed IL-17 family cytokine, found in lining but most strongly expressed in human neutrophil elastase containing polymorphonuclear cells. This pattern was distinct from that of IL-17A, which was found in mast cell tryptase immunoreactive cells. Circulating neutrophils contained IL-17B, verifying the in vivo results. Fibroblasts up-regulated the expression of IL-17RB, a putative receptor of IL-17B, after TNF-α stimulation. IL-17B significantly enhanced TNF-α-induced production of G-CSF and IL-6 in fibroblasts. Conclusion: IL-17B, which is present in synovium, may contribute to the pathogenesis of RA. IL-17B can enhance the effects of TNF-α on the production of cytokines and chemokines that control immune cell trafficking and neutrophil homeostasis in the inflamed tissues.
机译：目的：T辅助细胞17（Th17）和肥大细胞在RA中产生IL-17A，并在RA发病中起关键作用。但是，RA中完整的IL-17细胞因子谱尚不清楚。该研究的目的是系统地研究RA中IL-17家族细胞因子的表达。方法：采用定量RT-PCR（qRT-PCR）测定RA滑膜和血管pan以及OA滑膜中所有IL-17细胞因子的表达。 IL-17A和IL-17B被免疫染色。分析外周血中性粒细胞的IL-17B。体外测试了IL-17B单独或与TNF-α结合对成纤维细胞和内皮细胞的作用。结果：在所有组织中，IL-17B是表达最多的IL-17家族细胞因子，在细胞壁中发现，但在含有人中性粒细胞弹性蛋白酶的多形核细胞中表达最强。这种模式不同于在肥大细胞类胰蛋白酶免疫反应性细胞中发现的IL-17A。循环中性粒细胞含有IL-17B，验证了体内结果。 TNF-α刺激后，成纤维细胞上调了IL-17B的假定受体IL-17RB的表达。 IL-17B显着增强了成纤维细胞中TNF-α诱导的G-CSF和IL-6的产生。结论：滑膜中存在的IL-17B可能与RA的发病有关。 IL-17B可以增强TNF-α对控制发炎组织中免疫细胞运输和中性粒细胞稳态的细胞因子和趋化因子产生的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号