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首页> 外文期刊>RSC Advances >H2O2-mediated fluorescence quenching of double-stranded DNA templated copper nanoparticles for label-free and sensitive detection of glucose
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H2O2-mediated fluorescence quenching of double-stranded DNA templated copper nanoparticles for label-free and sensitive detection of glucose

机译：H2O2介导的双链DNA模板铜纳米颗粒的荧光猝灭，用于无标记和灵敏的葡萄糖检测

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摘要

Blood glucose monitoring has attracted extensive attention because diabetes mellitus is a worldwide public health problem. Here, a novel and label-free fluorescent sensing strategy was reported for simple and sensitive detection of glucose in human serum on the basis of H2O2-mediated fluorescence quenching of double-stranded DNA (ds-DNA) templated copper nanoparticles (Cu NPs). In this strategy, the fluorescence intensity of ds-DNA templated Cu NPs was found to be quenched effectively in the presence of H2O2. Similarly, glucose could be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Under the optimized conditions, the strategy exhibited sensitive and selective detection of glucose in a linear range from 50 nM to 100 mu M and with a detection limit of 12 nM. In addition, the method was successfully applied in the detection of glucose in human serum samples with satisfactory results. Furthermore, the strategy was free of any fluorescence dye label, complex DNA sequence design, and sophisticated experimental techniques. Therefore, the proposed approach could hold great potential for diabetes mellitus research and clinical diagnosis.
机译：血糖监测已引起广泛关注，因为糖尿病是世界范围内的公共卫生问题。在这里，基于H2O2介导的双链DNA（ds-DNA）模板铜纳米颗粒（Cu NPs）的H2O2介导的荧光猝灭，报道了一种新颖且无标记的荧光传感策略，用于简单而灵敏地检测人血清中的葡萄糖。在这种策略中，发现在H2O2存在下，ds-DNA模板化的Cu NPs的荧光强度被有效淬灭。类似地，可以基于葡萄糖通过葡萄糖氧化酶的酶促转化产生H 2 O 2来监测葡萄糖。在优化的条件下，该策略在50 nM至100μM的线性范围内显示了灵敏和选择性的葡萄糖检测，检测极限为12 nM。此外，该方法已成功用于人血清样品中葡萄糖的检测，结果令人满意。此外，该策略没有任何荧光染料标记，复杂的DNA序列设计和复杂的实验技术。因此，所提出的方法在糖尿病研究和临床诊断中具有巨大的潜力。

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