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Peptide arrays for detecting naphthenic acids in oil sands process affected water

机译：用于检测油砂工艺受影响水中环烷酸的肽阵列

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摘要

Naphthenic acids (NAs) are water-soluble components of petroleum. The characterization and quantification of NAs by analytical methods have proved quite challenging, whilst the toxic effects of these water-soluble compounds on a variety of organisms adversely affecting reproduction and steroid production is becoming apparent. In this study, we report a fluorescence-based competitive binding method for rapid sensing of the presence of NAs using cellulosic peptide array strips as sensors. The peptide array was designed from sequences derived from the estrogen receptor (ER). Several of these peptides were able to detect the presence of NAs at low micromolar (similar to 5 mg L-1) levels in different oil sands process affected water samples. The specific binding of one of the peptides, peptide 17 (EGXVEIFDXLLATS) with NAs was evaluated using isothermal titration calorimetry. The results show that peptide 17 interacts strongly with NAs with an apparent binding constant (K-a) of 96 x 10(6) M-1, and may bind NAs in a similar fashion as ER interacts with estrogen. Finally, the data support that the peptides displaying high affinity for NAs can be used for developing disposable peptide-based sensor arrays for NA detection in oil sands process affected water samples.
机译：环烷酸（NAs）是石油的水溶性成分。通过分析方法对NAs进行表征和定量已证明是具有挑战性的，而这些水溶性化合物对各种对繁殖和类固醇产生不利影响的生物的毒性作用已变得显而易见。在这项研究中，我们报告了一种基于荧光的竞争性结合方法，可使用纤维素肽阵列条作为传感器快速检测NA的存在。从源自雌激素受体（ER）的序列设计肽阵列。这些肽中的几种能够在低油摩尔（类似于5 mg L-1）水平下在不同的受油砂处理影响的水样品中检测到NAs的存在。使用等温滴定量热法评估一种肽，即肽17（EGXVEIFDXLLATS）与NA的特异性结合。结果表明，肽17与NA相互作用很强，其表观结合常数（K-a）为96 x 10（6）M-1，并且可能以与ER与雌激素相互作用相似的方式结合NA。最后，数据支持显示出对NAs具有高亲和力的肽可用于开发可丢弃的基于肽的传感器阵列，以在油砂工艺影响的水样中进行NA检测。

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