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首页> 外文期刊>RSC Advances >In vitro 30 nm silver nanoparticles promote chondrogenesis of human mesenchymal stem cells
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In vitro 30 nm silver nanoparticles promote chondrogenesis of human mesenchymal stem cells

机译：体外30 nm银纳米颗粒促进人间充质干细胞的软骨形成

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摘要

Silver nanoparticles (Ag NPs) are one of the most widely used products in nano-medicine due to their broad-spectrum antimicrobial activity. In tissue engineering, Ag NPs are often incorporated as antibacterial agents in scaffolds, which are subsequently loaded with human bone marrow-derived mesenchymal stem cells (hMSCs). In this study, we investigated the effect of Ag NPs on chondrogenesis of hMSCs. The synthesized Ag NPs were spherical in shape, with a mean diameter of similar to 30 nm. After 24 h exposure, Ag NPs were taken up into hMSCs and mainly distributed in the cytoplasm, the nucleus and different sized vesicles. We examined the chondrogenesis through several methods, including glycosaminoglycan (GAG) detection, immunohistochemical staining for type II collagen and aggrecan and real-time PCR for Sry-related high-mobility-group box 9 (SOX9), cartilage oligomeric matrix protein (COMP) and type X collagen. The present results indicate that long-term exposure to Ag NPs (at a concentration of 10 mu g mL(-1) and 20 mu g mL(-1)) led to an increased expression of SOX9, COMP and GAG, while type II collagen expression was unaffected. Short-term exposure to Ag NPs (at a concentration of 30 mu g mL(-1) and 40 mu g mL(-1)) resulted in a slight increase in SOX9 expression, while no change in GAG, aggrecan, type II collagen or COMP content was found. Expression of type X collagen, a marker for hypertrophic chondrocytes, was reduced after both long-and short-term exposure. In conclusion, 30 nm Ag NPs have positive effects on chondrogenesis since they can promote the expression of chondrogenic markers while reducing hypertrophy of hMSCs.
机译：银纳米颗粒（Ag NPs）由于其广谱抗菌活性而成为纳米药物中使用最广泛的产品之一。在组织工程中，Ag NPs通常作为抗菌剂掺入支架中，然后再向支架中加载人骨髓来源的间充质干细胞（hMSCs）。在这项研究中，我们调查了Ag NPs对hMSCs软骨形成的影响。合成的Ag NP呈球形，平均直径类似于30 nm。暴露24 h后，Ag NP被吸收到hMSC中，主要分布在细胞质，细胞核和不同大小的囊泡中。我们通过几种方法检查了软骨形成，包括糖胺聚糖（GAG）检测，II型胶原蛋白和聚集蛋白聚糖的免疫组织化学染色以及Sry相关的高迁移率族框9（SOX9），软骨寡聚基质蛋白（COMP）的实时PCR。和X型胶原蛋白。目前的结果表明，长期暴露于Ag NPs（浓度为10μg mL（-1）和20μg mL（-1））导致SOX9，COMP和GAG的表达增加，而II型胶原蛋白表达不受影响。短期暴露于Ag NPs（浓度分别为30μg mL（-1）和40μgmL（-1））导致SOX9表达略有增加，而GAG，聚集蛋白聚糖，II型胶原蛋白没有变化或找到COMP内容。长期和短期暴露后，肥大软骨细胞标记物X型胶原的表达降低。总之，30 nm Ag NPs对软骨形成有积极作用，因为它们可以促进软骨生成标志物的表达，同时减少hMSCs的肥大。

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