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首页> 外文期刊>RSC Advances >Protein binding and biological evaluation of a polymer-anchored cobalt(III) complex containing a 2,2 '-bipyridine ligand
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Protein binding and biological evaluation of a polymer-anchored cobalt(III) complex containing a 2,2 '-bipyridine ligand

机译：含2,2'-联吡啶配体的聚合物锚定钴（III）配合物的蛋白质结合和生物学评估

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摘要

The molecular interaction of polymer-anchored cobalt(III) complex, cis-[Co(bpy)(2)(BPEI)Cl]Cl-2 center dot 4H(2)O (bpy = 2,2'-bipyridine, BPEI = branched polyethyleneimine) with two plasma proteins, human serum albumin (HSA) and bovine serum albumin (BSA) using various spectrophotomeric techniques has been investigated. The steady-state and time-resolved fluorescence spectra clearly demonstrated the static quenching mechanism. The calculated thermodynamic parameters revealed that the interaction between the polymer-cobalt(III) complex and HSA/BSA was driven mainly by van der Waals forces and hydrogen bonds. The results observed from three dimensional fluorescence and circular dichorism (CD) spectral studies manifested the conformational changes of HSA/BSA upon addition of the polymer-cobalt(III) complex. Furthermore, the antimicrobial result showed that the polymer-cobalt(III) complex exhibits good antibacterial and antifungal activities against certain human pathogenic microorganisms. In addition, the antiproliferative properties of the polymer-cobalt (III) complex on the HEp-2 human larynx cancer cells were determined using the MTT assay. The mode of cell death induced by the complex following treatment was analyzed adopting specific staining techniques. MTT assay revealed that the viability of the cells thus treated was significantly decreased and the cells succumbed to apoptosis as well as necrosis as reflected in changes in the nuclear morphology and cytoplasmic features by AO & EB and Hoechst staining methods.
机译：聚合物锚定的钴（III）配合物，顺式-[Co（bpy）（2）（BPEI）Cl] Cl-2中心点4H（2）O的分子相互作用（bpy = 2,2'-联吡啶，BPEI =使用各种分光光度技术研究了含有两种血浆蛋白的人支化聚乙烯亚胺（人血清白蛋白（HSA）和牛血清白蛋白（BSA））。稳态和时间分辨荧光光谱清楚地证明了静态猝灭机理。计算的热力学参数表明，聚合物-钴（III）配合物与HSA / BSA之间的相互作用主要由范德华力和氢键驱动。从三维荧光和圆二色性（CD）光谱研究中观察到的结果表明，添加聚合物-钴（III）配合物后，HSA / BSA的构象变化。此外，抗菌结果表明，聚合物-钴（III）配合物对某些人类病原微生物表现出良好的抗菌和抗真菌活性。另外，使用MTT测定法确定了聚合物-钴（III）复合物在HEp-2人喉癌细胞上的抗增殖特性。采用特定的染色技术分析了复合物在处理后诱导的细胞死亡方式。 MTT分析表明，如此处理的细胞的活力显着降低，并且由于AO＆EB和Hoechst染色方法的核形态和细胞质特征的变化反映了细胞屈服于凋亡和坏死。

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