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首页> 外文期刊>RSC Advances >A sandwich-like electrochemiluminescent biosensor for the detection of concanavalin A based on a C-60-reduced graphene oxide nanocomposite and glucose oxidase functionalized hollow gold nanospheres
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A sandwich-like electrochemiluminescent biosensor for the detection of concanavalin A based on a C-60-reduced graphene oxide nanocomposite and glucose oxidase functionalized hollow gold nanospheres

机译：基于C-60还原氧化石墨烯纳米复合物和葡萄糖氧化酶功能化的空心金纳米球的夹心样电化学发光生物传感器，用于检测伴刀豆球蛋白A

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A sensitive sandwich-like electrochemiluminescent (ECL) biosensor was designed for the detection of concanavalin A (ConA) using a C-60-reduced graphene oxide (C-60-rGO) nanocomposite as a platform and glucose oxidase (GOX) decorated hollow gold nanospheres (HGNSs) as a label. First, C-60-rGO with a large surface area was prepared for combining with phenoxy-derivatized dextran, which served as the recognition element for interacting with ConA by biospecific carbohydrate-protein (lectin) interactions. Then, GOX decorated HGNSs (GOX-HGNSs) were linked to the electrode surface through the biospecific interaction between the intrinsic carbohydrate residues of GOX and ConA. These localized GOX and HGNSs amplified the ECL signal of luminol intensely, which was achieved by the efficient catalysis of the GOX towards the oxidation of glucose to in situ generate an improved amount of hydrogen peroxide (H2O2) as a coreactant, and the excellent catalysis of HGNSs towards the ECL reaction of luminol-H2O2. The prepared biosensor exhibited a sensitive response for the determination of ConA, ranging from 0.10 to 100 ng mL(-1) with a detection limit down to 30 pg mL(-1) (signal to noise = 3). With excellent stability, sensitivity, selectivity and simplicity, the prepared biosensor showed great prospects in lectin sensing or carbohydrate sensing.
机译：设计了灵敏的三明治状电化学发光（ECL）生物传感器，用于以C-60还原氧化石墨烯（C-60-rGO）纳米复合材料为平台和葡萄糖氧化酶（GOX）装饰的中空金来检测伴刀豆球蛋白A（ConA）。纳米球（HGNS）作为标签。首先，制备具有大表面积的C-60-rGO，以与苯氧基衍生的葡聚糖结合，后者作为通过生物特异性碳水化合物-蛋白质（凝集素）相互作用与ConA相互作用的识别元件。然后，GOX装饰的HGNS（GOX-HGNS）通过GOX和ConA的固有碳水化合物残基之间的生物特异性相互作用与电极表面连接。这些局部的GOX和HGNS强烈增强了鲁米诺的ECL信号，这是通过有效催化GOX催化葡萄糖氧化以原位生成改进量的过氧化氢（H2O2）作为共反应剂而实现的，并获得了出色的催化作用。 HGNS参与Luminol-H2O2的ECL反应。制备的生物传感器对ConA的测定显示出灵敏的响应，范围从0.10到100 ng mL（-1），检测限低至30 pg mL（-1）（信噪比= 3）。所制备的生物传感器具有优异的稳定性，灵敏度，选择性和简便性，在凝集素传感或碳水化合物传感方面显示出广阔的前景。

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