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首页> 外文期刊>RSC Advances >An electrochemical sensing strategy for the detection of the hepatitis B virus sequence with homogenous hybridization based on host-guest recognition
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An electrochemical sensing strategy for the detection of the hepatitis B virus sequence with homogenous hybridization based on host-guest recognition

机译：基于宿主-客体识别的同源杂交的乙型肝炎病毒序列电化学检测策略

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摘要

Rapid, sensitive, and accurate DNA detection is of great significance to meet the growing demand of disease diagnostics. Herein, an ultrasensitive and highly specific electrochemical sensing strategy for detection of hepatitis B virus (HBV) DNA hybridization in homogeneous solution was presented. In this sensing protocol, magnetic nanoparticles (MNPs) were prepared and modified with beta-CD. MNPs/beta-CD were then used for host-guest recognition, separation and detection. In the experiment, a stem-loop structured DNA was designed as the probe to indicate the occurrence of hybridization. It was labeled with dabcyl at the 5'-end as a guest molecule and Au nanoparticles at the 3'-end as an electrochemical tag. Initially, the DNA probe retained the stem-loop configuration, which shielded dabcyl from docking with beta-CD/MNP in solution due to the steric effect. While in the presence of the complementary target DNA, the stem-loop structure of the probe was dissociated and the double-stranded DNA (dsDNA) was formed as a result of the hybridization. Consequently, dsDNA was linked to beta-CD/MNP owing to the host-guest recognition between b-CD and dabcyl. Thus, the hybridization events could be sensitively transduced to electrochemical signals provided by Au nanoparticles. Au nanoparticles were then captured by the electrode through an external magnetic field. The designed sensor favored discrimination between the healthy and single-nucleotide polymorphism (SNP)-containing sequences. Under the optimized detection conditions, the sensing strategy showed high sensitivity and the detection limit was down to 9.930 x 10(-13) M for a complementary HBV DNA sequence.
机译：快速，灵敏和准确的DNA检测对于满足不断增长的疾病诊断需求具有重要意义。在这里，提出了一种用于检测乙型肝炎病毒（HBV）DNA杂交均匀溶液中的超灵敏和高度特异性的电化学传感策略。在该传感方案中，制备了磁性纳米颗粒（MNP），并用β-CD修饰。然后将MNP /β-CD用于客体识别，分离和检测。在实验中，茎环结构的DNA被设计为探针，以指示杂交的发生。它在5'端标记为dabcyl作为来宾分子，在3'端标记为Au纳米颗粒作为电化学标签。最初，DNA探针保留了茎环构型，由于空间效应，该构型阻止了dabcyl与溶液中的β-CD/ MNP对接。当存在互补靶DNA时，探针的茎环结构被解离，并且由于杂交而形成了双链DNA（dsDNA）。因此，由于b-CD和dabcyl之间的客体识别，dsDNA与β-CD/ MNP连接。因此，杂交事件可以灵敏地转导至Au纳米颗粒提供的电化学信号。然后电极通过外部磁场捕获金纳米颗粒。设计的传感器有利于区分健康序列和包含单核苷酸多态性（SNP）的序列。在优化的检测条件下，感测策略显示出高灵敏度，并且互补HBV DNA序列的检测极限低至9.930 x 10（-13）M。

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