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首页> 外文期刊>RSC Advances >Synthesis, characterization and biological evaluation of chromen and pyrano chromen-5-one derivatives impregnated into a novel collagen based scaffold for tissue engineering applications
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Synthesis, characterization and biological evaluation of chromen and pyrano chromen-5-one derivatives impregnated into a novel collagen based scaffold for tissue engineering applications

机译：铬和吡喃铬5-酮衍生物的合成，表征及生物学评价，该衍生物浸渍在用于组织工程的新型胶原基支架中

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In this article, we describe the synthesis and biological evaluation of a novel 2-(methylamino)-3-nitro-4-(4-oxo- 4H-chromen-3-yl) pyrano[3,2-c]chromen-5(4H)-one (CCN). It is also determined that CCN impregnated into the collagen scaffold has the potential to mimic the function of the extracellular matrix as a biomaterial in the field of tissue engineering. The series of pyrano[3,2-c] chromen-5(4H)-one derivatives (4a-4j), was analyzed by H-1 NMR, C-13 NMR, mass spectra and FTIR analysis. Compound 4c was confirmed by single crystal XRD studies. All the compounds were screened for antimicrobial activity against Gram positive, Gram negative bacteria and yeast. Among all the compounds, compound (4a=CCN) showed activity against Gram positive and Gram negative bacteria, when compared to the synthesized compounds. Further, compound CCN was evaluated for cytotoxicity against MCF-7, Hep-2 and Vero cancer cell lines with IC50 values of 5.4 mu g ml(-1), 5.3 mu g ml(-1) and 68.4 mu g ml(-1) respectively. In addition, the results of flow cytometry and docking (PDBID: 1A27 with the ligand) studies supported the activity of the synthesized compound (4a). FTIR and NMR analysis of the CCN impregnated collagen scaffold were done to reveal the existence of the CCN molecule in the scaffold. The inherent property of the collagen scaffold was not significantly affected by the structure of the CCN molecule. The thermal and mechanical properties of the collagen scaffold impregnated with CCN molecules gives stability as well as supports the swelling. However, the COL-CCN scaffold showed an enhanced cell attachment and proliferation of NIH 3T3 fibroblast cells. Based on the results, the novel CCN molecule impregnated within a collagen scaffold has potential application as a biomaterial in tissue engineering.
机译：在本文中，我们描述了新型2-（甲基氨基）-3-硝基-4-（4-氧代-4H-chromen-3-yl）吡喃并[3,2-c] chromen-5的合成和生物学评价（4H）-一（CCN）。还确定了浸渍到胶原蛋白支架中的CCN具有模仿组织工程领域中作为生物材料的细胞外基质功能的潜力。通过H-1 NMR，C-13 NMR，质谱和FTIR分析，分析了一系列吡喃并[3,2-c] chromen-5（4H）-一衍生物（4a-4j）。通过单晶XRD研究证实了化合物4c。筛选所有化合物对革兰氏阳性，革兰氏阴性细菌和酵母的抗菌活性。在所有化合物中，与合成的化合物相比，化合物（4a = CCN）对革兰氏阳性菌和革兰氏阴性菌表现出活性。此外，评估了化合物CCN对MCF-7，Hep-2和Vero癌细胞系的细胞毒性，IC50值分别为5.4μg ml（-1），5.3μgml（-1）和68.4μgml（-1） ） 分别。另外，流式细胞术和对接（具有配体的PDBID：1A27）研究的结果支持了合成​​化合物（4a）的活性。对CCN浸渍的胶原蛋白支架进行FTIR和NMR分析，以揭示支架中CCN分子的存在。胶原蛋白支架的固有性质不受CCN分子结构的显着影响。浸渍有CCN分子的胶原蛋白支架的热和机械性能既稳定又支持肿胀。然而，COL-CCN支架显示出增强的细胞附着和NIH 3T3成纤维细胞的增殖。根据结果​​，浸渍在胶原蛋白支架中的新型CCN分子作为生物材料在组织工程中具有潜在的应用。

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