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首页> 外文期刊>RSC Advances >Synthesis and X-ray structure of a new zinc(II) coordination polymer: interaction with DNA and double stranded RNA and elucidation of the molecular aspects of the binding to bovine serum albumin
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Synthesis and X-ray structure of a new zinc(II) coordination polymer: interaction with DNA and double stranded RNA and elucidation of the molecular aspects of the binding to bovine serum albumin

机译：新型锌（II）配位聚合物的合成和X射线结构：与DNA和双链RNA的相互作用以及与牛血清白蛋白结合的分子方面的阐明

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摘要

A novel ligand bridged zinc(II) coordination polymer, [Zn(4-Me-5-CHOIm)(2)(HCOO)](ClO4) has been synthesized and characterized by single crystal X-ray diffraction studies. The structural analysis revealed that the complex exhibits two types of 2D network by weak and strong hydrogen bonds. Interestingly, an extended supramolecular layer network has been observed in the solid state structure through weak face-to-face pi-stacking interactions. The interaction of the complex with calf thymus DNA (CT-DNA) and double stranded ribonucleic acid, polyinosinic center dot polycytidylic acid (poly(I)center dot poly(C)) has been explored by UV-Vis absorption, fluorescence displacement, circular dichroism (CD) spectral and isothermal titration calorimetric (ITC) methods. The spectral titration method revealed that the compound could interact with CT-DNA and RNA through partial intercalation with a binding affinity of an order of 10(5). ITC experiments indicate an exothermic reaction in both cases but RNA interaction is favored by positive entropy. On the other hand, the complex could quench the intrinsic fluorescence of BSA in a static quenching process with a quenching constant of the order of 10(4). A specific binding distance of 2.75 nm between Trp 212 (donor) and the complex (acceptor) was obtained from Forster resonance energy transfer studies (FRET). Competitive binding using site markers, warfarin and ibuprofen, having definite binding sites demonstrated that the complex binds to site I (subdomain IIA) on BSA. Circular dichroism (CD), synchronous fluorescence, and three-dimensional fluorescence spectroscopy experiments reveal that binding with the complex alters the BSA secondary structure by a reduction of the alpha-helical organization and increases the coiled structure and the ITC experiment indicates that the binding was enthalpy driven with a slight negative entropy effect.
机译：合成了一种新型的配体桥联锌（II）配位聚合物[Zn（4-Me-5-CHOIm）（2）（HCOO）]（ClO4），并通过单晶X射线衍射研究对其进行了表征。结构分析表明，该复合物通过弱和强氢键表现出两种类型的二维网络。有趣的是，已经通过微弱的面对面pi堆积相互作用在固态结构中观察到扩展的超分子层网络。该复合物与小胸腺DNA（CT-DNA）和双链核糖核酸，多肌苷中心点聚胞苷酸（poly（I）center dot poly（C））的相互作用已通过UV-Vis吸收，荧光位移，圆形二色性（CD）光谱和等温滴定量热（ITC）方法。光谱滴定法表明该化合物可通过部分插层与CT-DNA和RNA相互作用，结合亲和力约为10（5）。 ITC实验表明，在两种情况下均发生放热反应，但正熵有利于RNA相互作用。另一方面，该络合物可以在静态猝灭过程中猝灭BSA的固有荧光，猝灭常数约为10（4）。从Forster共振能量转移研究（FRET）获得了Trp 212（供体）和复合物（受体）之间2.75 nm的特定结合距离。使用具有明确结合位点的位点标记华法林和布洛芬进行竞争性结合，证明该复合物与BSA上的位点I（亚域IIA）结合。圆二色性（CD），同步荧光和三维荧光光谱实验表明，与配合物的结合通过减少α-螺旋结构改变了BSA二级结构，并增加了螺旋结构，ITC实验表明这种结合是焓驱动，具有轻微的负熵效应。

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