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Preparation of luminescent titania/dye hybrid nanoparticles and their dissolution properties for controlling cellular environments

机译：发光二氧化钛/染料杂化纳米粒子的制备及其对细胞环境的控制

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To design non-cytotoxic controlled release system is one of the major challenges in such a field as biomedicine. For this purpose, well-defined, luminescent and cytocompatible titania/dye hybrid nanoparticles (NPs) are synthesized. Hybrid NPs with a uniform size/shape are achieved through the precisely controlled microfluidic hydrolysis and co-condensation reactions of titanium tetraisopropoxide and 3-aminopropyltriethoxysilane in the presence of octadecylamine (ODA). By adding the cell-staining luminescent dye of fluorescein isothiocyanate (FITC) molecules to the starting solution, the spherical titania/aminopropyl-silica/ODA/FITC hybrid NPs (average diameter: 400 nm, coefficient of variation: 9.5%) are successfully prepared. Interestingly, the NPs demonstrate enhanced luminescence after treating them with phosphate buffered saline (PBS) and a possible mechanism is discussed. These NPs are not dispersed in PBS during the treatment due to their hydrophobic surface, which is an important feature to avoid cytotoxicity caused by an endocytic pathway. The NIH3T3 fibroblast viability test revealed that the hybrid NPs are not cytotoxic, realizing the clear imaging of the cellular shapes by the cellular gradual reactions mainly with the FITC released from the hybrid NPs floating at the interface. Therefore, the well-defined hybrid NPs with tailored surface/release properties are promising candidates for slowly-controlling cellular environmental systems (e.g., drug delivery systems).
机译：设计无细胞毒性的控释系统是生物医学等领域的主要挑战之一。为此目的，合成了定义明确的，发光的和细胞相容性的二氧化钛/染料杂化纳米颗粒（NP）。通过在十八烷基胺（ODA）存在下，四异丙醇钛和3-氨基丙基三乙氧基硅烷的精确控制的微流体水解和共缩合反应，可以实现具有均匀大小/形状的杂化NP。通过将异硫氰酸荧光素（FITC）分子的细胞染色发光染料添加到起始溶液中，成功制备了球形二氧化钛/氨基丙基-二氧化硅/ ODA / FITC杂化NP（平均直径：400 nm，变异系数：9.5％） 。有趣的是，NPs在用磷酸盐缓冲盐水（PBS）处理后显示出增强的发光，并讨论了可能的机制。这些NP由于其疏水表面而在治疗期间未分散在PBS中，这是避免由内吞途径引起的细胞毒性的重要特征。 NIH3T3成纤维细胞的生存能力测试表明，杂化NP没有细胞毒性，主要通过从漂浮在界面上的杂化NP释放的FITC的细胞逐渐反应，实现了细胞形状的清晰成像。因此，具有定制的表面/释放特性的界限分明的杂合NP是用于缓慢控制细胞环境系统（例如，药物递送系统）的有希望的候选者。

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