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首页> 外文期刊>RSC Advances >Liposomes equipped with poly(N-isopropyl acryl amide)-containing coatings as potential drug carriers
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Liposomes equipped with poly(N-isopropyl acryl amide)-containing coatings as potential drug carriers

机译：配备有含聚（N-异丙基丙烯酰胺）涂层的脂质体作为潜在的药物载体

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Polymer coated liposomes are promising drug delivery candidates. Herein, we report on the coating of liposomes with mixed films containing poly(dopamine) (PDA) and different types of poly(N-isopropyl acryl amide) (pNiPAAm). Their potential as drug carriers from solution in the presence of shear stress or in a substrate-mediated manner using macrophages is assessed. Although we found no difference in the cell mean fluorescence (CMF) when applying shear stress when employing fluorescently labeled liposomes, macrophages exposed to liposomes coated with a mixture of PDA and highly-branched pNiPAAm (L-D/HB) exhibited significantly higher CMF after 2.5 h compared to liposomes coated with only PDA (L-D) or with a mixture of PDA and aminated pNiPAAm (L-2(D/pNH)). The coated liposomes did not affect the cell viability in the time frame tested, but the application of shear stress reduced the number of surface-adherent macrophages. L-D, L-2(D/pNH) or L-D/pHB could be immobilized to poly(L lysine) pre-coated silica substrates. All substrates were equally suited for macrophages to adhere. The CMF of the adhering macrophages was found to be independent of the coating of the surface-immobilized liposomes when using fluorescently labeled liposomes in the coatings. Taken together, we demonstrate that mixed PDA-based coatings can be used to affect the interaction of liposomes with macrophages with potential in drug delivery.
机译：聚合物包衣的脂质体是有希望的药物递送候选物。在本文中，我们报道了脂质体与包含聚（多巴胺）（PDA）和不同类型的聚（N-异丙基丙烯酰胺）（pNiPAAm）的混合膜的涂层。在存在剪切应力的情况下，或使用巨噬细胞以底物介导的方式，评估了它们作为溶液中药物载体的潜力。尽管在使用荧光标记脂质体时施加剪切应力时，我们发现细胞平均荧光（CMF）没有差异，但是暴露于涂有PDA和高度支化pNiPAAm（LD / HB）混合物的脂质体的巨噬细胞在2.5 h后显示出明显更高的CMF与仅涂有PDA（LD）或涂有PDA和胺化pNiPAAm（L-2（D / pNH））的混合物的脂质体相比。包被的脂质体在测试的时间范围内不影响细胞活力，但是施加剪切应力减少了表面粘附的巨噬细胞的数量。 L-D，L-2（D / pNH）或L-D / pHB可以固定在聚（L赖氨酸）预涂的二氧化硅基材上。所有底物均同样适合巨噬细胞粘附。当在涂层中使用荧光标记的脂质体时，发现粘附的巨噬细胞的CMF独立于表面固定的脂质体的涂层。两者合计，我们证明混合的基于PDA的涂层可用于影响脂质体与巨噬细胞的相互作用，具有潜在的药物传递作用。

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