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首页> 外文期刊>RSC Advances >A rapid fluorescence detecting platform: applicable to sense carnitine and chloramphenicol in food samples
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A rapid fluorescence detecting platform: applicable to sense carnitine and chloramphenicol in food samples

机译：快速的荧光检测平台：适用于检测食品样品中的肉碱和氯霉素

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摘要

A new coenzyme A (CoA) fluorescence platform has been described for the sensitive determination of CoA, carnitine and chloramphenicol. Previously, our research group reported a long-wavelength latent florescent probe, termed "BCC". Here, we extend the application of BCC as an off-on fluorescence ratiometric indicator for the detection of acetyl CoA with a linear range of 0.5-10 mu M and limit of detection (LOD) of 0.18 mu M. We established a novel platform for the determination of carnitine and chloramphenicol in the presence of acetyl-CoA and their corresponding acetyl-CoA-transferring enzymes (chloramphenicol acetyltransferase (CAT) and carnitine acetyltransferase (CrAT), respectively). The proposed method detects carnitine and chloramphenicol in the linear range of 0.5-10 mu M. The LODs for the determination of carnitine and chloramphenicol were found to be 0.5 mu M and 0.3 mu M, respectively. Moreover, BCC provides a facile assay platform for real-time monitoring of CAT and CrAT enzymatic activity in the presence of their corresponding substrates and acetyl-CoA. Practical feasibility of the proposed method has been demonstrated in food samples such as milk, powdered milk and honey, and the observed appreciable recoveries revealed its promising practicality.
机译：已经描述了一种新的辅酶A（CoA）荧光平台，用于灵敏测定CoA，肉碱和氯霉素。以前，我们的研究小组报告了一种长波长潜伏荧光探针，称为“ BCC”。在这里，我们扩展了BCC作为线性荧光比率指示剂的应用，用于线性范围为0.5-10μM和检测限（LOD）为0.18μM的乙酰辅酶A检测。我们建立了一个新的平台在乙酰辅酶A及其相应的乙酰辅酶A转移酶（分别为氯霉素乙酰转移酶（CAT）和肉碱乙酰转移酶（CrAT））存在下测定肉碱和氯霉素。所提出的方法可检测线性范围为0.5-10μM的肉碱和氯霉素。测定肉碱和氯霉素的LOD分别为0.5μM和0.3μM。此外，BCC提供了一种简便的测定平台，可在存在相应底物和乙酰辅酶A的情况下实时监测CAT和CrAT的酶活性。该方法在牛奶，奶粉和蜂蜜等食品样品中已证明了其可行性，并且观察到的明显回收率表明了该方法的实用性。

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