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Livestock-associated MRSA ST398 carriage in pig slaughterhouse workers related to quantitative environmental exposure

机译:猪屠宰场工人与牲畜相关的MRSA ST398运输与定量环境暴露有关

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Objectives: To assess livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) carriage among workers in pig slaughterhouses and assess associated risk factors, including occupational exposure to LA-MRSA. Methods: A cross-sectional study in three Dutch pig slaughterhouses was undertaken. Nasal swabs of participants were taken. Nasal swabs and surface wipes, air and glove samples were screened for presence of methicillin-resistant Staphylococcus aureus (MRSA). MRSA was quantitatively determined on gloves and in air samples by culturing and real-time PCR. Results: 11 of 341 (3.2%) participants were identified as nasal MRSA carriers. MRSA-positive workers were predominantly found at the start of the slaughter process. Major risk factors for carriage were working in the lairage and working in the scalding and dehairing area. Most nasal isolates (73%) belonged to the LA-MRSA clone ST398. MRSA ST398-positive environmental samples were found throughout the slaughter process. A clear decrease was seen along the slaughterline in the number of MRSA-positive samples and in the MRSA amount per sample. Conclusions: This study showed that working in the lairage area or scalding and dehairing area were the major risk factors for MRSA carriage in pig slaughterhouse workers, while the overall prevalence of MRSA carriage is low. Occupational exposure to MRSA decreased along the slaughterline, and the risk of carriage showed a parallel decrease.
机译:目的:评估猪屠宰场工人中与牲畜相关的耐甲氧西林金黄色葡萄球菌(LA-MRSA)的运输,并评估相关的危险因素,包括职业性接触LA-MRSA的风险。方法:在三个荷兰猪屠宰场进行了横断面研究。采取参与者的鼻拭子。筛选鼻拭子和表面湿巾,空气和手套样品中是否存在耐甲氧西林的金黄色葡萄球菌(MRSA)。通过培养和实时PCR在手套和空气样品中定量测定MRSA。结果:341名参与者中有11名(3.2%)被确定为鼻MRSA携带者。 MRSA阳性工人主要是在屠宰过程开始时发现的。运送的主要危险因素是在平地里工作以及在烫伤和脱毛区域工作。大多数鼻分离物(73%)属于LA-MRSA克隆ST398。在整个屠宰过程中发现了MRSA ST398阳性的环境样品。沿着屠宰线,MRSA阳性样品的数量和每个样品的MRSA数量明显减少。结论:这项研究表明,在猪屠宰场工人在隔离区或烫伤和脱毛区工作是进行MRSA搬运的主要危险因素,而MRSA搬运的总体患病率较低。 MRSA的职业接触沿屠宰线减少,运输风险呈平行下降趋势。

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