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首页> 外文期刊>Revue de Medecine Veterinaire >Isolation, identification and molecular characterization of Brucella strains isolated from small ruminants in the West Bank, Palestine
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Isolation, identification and molecular characterization of Brucella strains isolated from small ruminants in the West Bank, Palestine

机译：巴勒斯坦西岸小反刍动物布鲁氏菌菌株的分离，鉴定和分子鉴定

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摘要

The seroprevalence of brucellosis in the small ruminant (sheep and goat) population from infected flocks was investigated in the West Bank (Palestine). 36.8% (129/350) and 32.3% (113/350) of animals were found to be positive by the Rose Bengal test (RBT) and the complement fixation test (CFT) respectively, the agreement score between the 2 methods being 89.1 %. The presence of Brucella directly assessed by bacterial isolation and PCR assay in milk samples from 80 seropositive animals was confirmed in47.5% (38/80) and in 77.5% (62/80) of samples, leading to a moderate agreement score between the 2 methods (70.0%). The phylogenic analysis of a putative open reading frame (ORF) in the haemagglutinin gene revealed that the current B. meliiensis isolates were genetically close to those from different Mediterranean countries (France, Spain and Cyprus) and particularly to the Israel strain. This first report of DNA sequencing of Brucella strains in small ruminants in Palestine would greatly help to implement control and preventive measures for brucellosis.
机译：在约旦河西岸（巴勒斯坦）调查了来自受感染鸡群的小反刍动物（绵羊和山羊）种群中布鲁氏菌病的血清阳性率。玫瑰孟加拉试验（RBT）和补体固定试验（CFT）分别发现36.8％（129/350）和32.3％（113/350）的动物为阳性，两种方法之间的一致性得分为89.1％ 。通过细菌分离和PCR分析直接评估了80种血清阳性动物的牛奶样品中布鲁氏菌的存在，分别在47.5％（38/80）和77.5％（62/80）的样品中得到了证实，从而导致布鲁氏菌之间的一致性得分中等2种方法（70.0％）。对血凝素基因推定阅读框（ORF）的系统发育分析表明，当前的B. meliiensis分离株在遗传上接近于来自不同地中海国家（法国，西班牙和塞浦路斯）的分离株，尤其是以色列菌株。巴勒斯坦小反刍动物中布鲁氏菌菌株DNA测序的第一份报告将极大地帮助实施布鲁氏菌病的控制和预防措施。

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