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首页> 外文期刊>Biomaterials >Cationic star polymers consisting of alpha-cyclodextrin core and oligoethylenimine arms as nonviral gene delivery vectors.
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Cationic star polymers consisting of alpha-cyclodextrin core and oligoethylenimine arms as nonviral gene delivery vectors.

机译：阳离子星形聚合物，由α-环糊精核心和寡乙烯亚胺臂组成，作为非病毒基因传递载体。

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摘要

A series of novel cationic star polymers were synthesized by conjugating multiple oligoethylenimine (OEI) arms onto an alpha-cyclodextrin (alpha-CD) core as nonviral gene delivery vectors. The molecular structures of the alpha-CD-OEI star polymers, which contained linear or branched OEI arms with different chain lengths ranging from 1 to 14 ethylenimine units, were characterized by using size exclusion chromatography, 13C and 1H NMR, and elemental analysis. The alpha-CD-OEI star polymers were studied in terms of their DNA binding capability, formation of nanoparticles with plasmid DNA (pDNA), cytotoxicity, and gene transfection in cultured cells. All the alpha-CD-OEI star polymers could inhibit the migration of pDNA on agarose gel through formation of complexes with pDNA, and the complexes formed nanoparticles with sizes ranging from 100 to 200 nm at N/P ratios of 8 or higher. The star polymers displayed much lower in vitro cytotoxicity than that of branched polyethylenimine (PEI) of molecular weight 25K. The alpha-CD-OEI star polymers showed excellent gene transfection efficiency in HEK293 and Cos7 cells. Generally, the transfection efficiency increased with an increase in the OEI arm length. The star polymers with longer and branched OEI arms showed higher transfection efficiency. The best one of the star polymers for gene delivery showed excellent in vitro transfection efficiency that was comparable to or even higher than that of branched PEI (25K). The novel alpha-CD-OEI star polymers with OEI arms of different chain lengths and chain architectures can be promising new nonviral gene delivery vectors with low cytotoxicity and high gene transfection efficiency for future gene therapy applications.
机译：通过将多个寡乙烯亚胺（OEI）臂缀合到α-环糊精（α-CD）核上作为非病毒基因递送载体，合成了一系列新型阳离子星形聚合物。通过尺寸排阻色谱，13C和1H NMR以及元素分析，对α-CD-OEI星型聚合物的分子结构进行了表征，该聚合物包含线性或分支的OEI臂，链长在1至14个亚乙基亚胺单元之间。研究了α-CD-OEI星型聚合物的DNA结合能力，带有质粒DNA（pDNA）的纳米颗粒的形成，细胞毒性以及在培养细胞中的基因转染。所有的α-CD-OEI星型聚合物都可以通过与pDNA形成复合物来抑制pDNA在琼脂糖凝胶上的迁移，并且该复合物形成的纳米颗粒的N / P比率为8或更高时，粒径范围为100至200 nm。与分子量为25K的支链聚乙烯亚胺（PEI）相比，星形聚合物的体外细胞毒性要低得多。 α-CD-OEI星型聚合物在HEK293和Cos7细胞中显示出出色的基因转染效率。通常，转染效率随OEI臂长的增加而增加。具有更长和分支的OEI臂的星形聚合物显示出更高的转染效率。最好的星形聚合物之一用于基因传递，表现出优异的体外转染效率，该效率可与分支PEI（25K）相比甚至更高。具有不同链长和链结构的OEI臂的新型α-CD-OEI星型聚合物可以成为有前途的新型非病毒基因传递载体，具有低细胞毒性和高基因转染效率，可用于未来的基因治疗应用。

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