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首页> 外文期刊>Cellular Signalling >Evidence for stimulation of adenylyl cyclase by an activated G(s) heterotrimer in cell membranes - An experimental method for controlling the G(s) subunit composition of cell membranes
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Evidence for stimulation of adenylyl cyclase by an activated G(s) heterotrimer in cell membranes - An experimental method for controlling the G(s) subunit composition of cell membranes

机译：激活的细胞膜中G（s）异源三聚体刺激腺苷酸环化酶的证据-控制细胞膜G（s）亚基组成的实验方法

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Heterotrimeric (alpha beta gamma) G(s) mediates agonist-induced stimulation of adenylyl cyclase (AC). Cholera toxin (CTx) will ADP-ribosylate the alpha-subunit of G(s) (G(s)alpha). G(s)alpha-deficient cyc(-) membranes were "stripped" of GP. When the stripped cyc(-) were incubated with G(s)alpha and/or G beta gamma, each was incorporated into the membranes independently of the other. Both G(s)alpha and G beta gamma had to be present in the membranes, and they had to be able to form a heterotrimer in order for CTx to ADP-ribosylate G(s)alpha, indicating that the membrane bound G(s) heterotrimer is a substrate for CTx, but the G(s)alpha subunit by itself is not. When G(s)alpha was completely and irreversibly activated with GTP gamma S and incorporated into stripped cyc(-), it was a poor substrate for CTx and a weak stimulator of AC unless G beta gamma was also incorporated. Furthermore, the level of AC stimulation corresponded to the amount of G(s) heterotrimer that was formed in the membranes from GTP gamma S-activated G(s)alpha and G beta gamma. These data suggest that AC is stimulated by an activated G(s) heterotrimer in cell membranes. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 30]
机译：异三聚体（αβγG）介导激动剂诱导的腺苷酸环化酶（AC）刺激。霍乱毒素（CTx）将ADP-核糖基化G（s）（G（s）alpha）的alpha亚基。 G（s）alpha缺陷的cyc（-）膜被“剥离”了GP。当将汽提的cyc（-）与G（s）alpha和/或G betaγ一起孵育时，彼此独立地掺入膜中。 G（s）alpha和G betaγ都必须存在于膜中，并且它们必须能够形成异源三聚体，以便CTx能够ADP-核糖基化G（s）alpha，这表明膜结合了G（s） ）异三聚体是CTx的底物，但G（s）alpha亚基本身不是。当G（g）alpha被GTPγS完全不可逆地激活并掺入剥离的cyc（-）中时，它是CTx的底物较差，而AC的刺激性很弱，除非还加入了Gβγ。此外，AC刺激的水平对应于由GTPγS活化的G（s）α和Gβγ在膜中形成的G（s）异三聚体的量。这些数据表明AC被细胞膜中活化的G（s）异源三聚体刺激。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：30]

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