...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Signalling >Toward a comprehensive characterization of the phosphotyrosine proteome
【24h】

Toward a comprehensive characterization of the phosphotyrosine proteome

机译：全面表征磷酸酪氨酸蛋白质组

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tyrosine phosphorylation (pTyr) regulates important cell functions and plays a key role in carcinogenesis. The purpose of this study was to perform a comprehensive study of the phosphotyrosine proteome. Immunoaffinity enriched pTyr proteins and peptides from K562 leukemia cells were analyzed with high-resolving liquid chromatography mass spectrometry. Two different antibodies selective for the pTyr modification were used in repeated enrichments to identify as many pTyr peptides as possible. Stringent verification of putative pTyr sites was performed to assure high reliability in the subsequent biological interpretation of the data. Identified pTyr proteins were subjected to pathway analysis by using different analytical tools. In total, 294 pTyr peptides belonging to 217 pTyr proteins were identified, 15 of which had not previously been reported to be modified by pTyr. The pTyr proteins were clustered in six major groups based on the biological functions "cellular signaling", "cell motility and shape", "cell cycle process", "transport", "RNA processing" and "protein processing". The pTyr proteins were mainly positioned in the following cellular compartments: cytoplasm, cytoskeleton, nucleus and ribonucleoprotein complexes. An interesting finding was that many proteins were related to RNA processing and were found to be heterogeneous nuclear ribonucleoproteins. Also, more than half of the novel pTyr proteins were localized to the nucleus, of which three (PBX2, TEAD1 and DIDO1) were classified as transcription factors and two (CENPC1 and MAD2L1) are associated with cell division control.
机译：酪氨酸磷酸化（pTyr）调节重要的细胞功能，并在致癌作用中起关键作用。这项研究的目的是对磷酸酪氨酸蛋白质组进行全面的研究。使用高分辨率液相色谱质谱仪分析了来自K562白血病细胞的免疫亲和力丰富的pTyr蛋白和多肽。对pTyr修饰具有选择性的两种不同抗体用于重复富集，以鉴定尽可能多的pTyr肽。对推定的pTyr位点进行了严格的验证，以确保在随后的数据生物学解释中具有很高的可靠性。通过使用不同的分析工具，对鉴定出的pTyr蛋白进行途径分析。总共鉴定出294种pTyr肽，它们属于217种pTyr蛋白，其中15种以前没有被pTyr修饰的报道。根据生物学功能“细胞信号传导”，“细胞运动性和形状”，“细胞周期过程”，“转运”，“ RNA加工”和“蛋白质加工”，将pTyr蛋白分为六个主要组。 pTyr蛋白主要位于以下细胞区室：细胞质，细胞骨架，细胞核和核糖核蛋白复合物。一个有趣的发现是，许多蛋白质与RNA加工有关，并且被发现是异质核核糖核蛋白。此外，一半以上的新型pTyr蛋白位于细胞核中，其中三个（PBX2，TEAD1和DIDO1）被分类为转录因子，另外两个（CENPC1和MAD2L1）与细胞分裂控制相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号