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首页> 外文期刊>Oncology: International Journal of Cancer Research and Treatment >Establishment and characterization of a new cell line (SKS) from neuroendocrine small cell carcinoma of the uterine cervix and its chemosensitivity.
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Establishment and characterization of a new cell line (SKS) from neuroendocrine small cell carcinoma of the uterine cervix and its chemosensitivity.

机译：子宫颈神经内分泌小细胞癌的新细胞系（SKS）的建立和表征及其化学敏感性。

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摘要

A new cell line (SKS) established from ascites of a patient with neuroendocrine small cell carcinoma of the uterine cervix had a good tumorigenicity and caused marked peritoneal dissemination, and was also highly sensitive to gemcitabine in an in vitro chemosensitivity test. SKS cells were small round cells with a high nuclear/cytoplasmic (N/C) ratio and grew into colony-like aggregates, forming spherical aggregates of floating cells. The population doubling time was 44 h. The number of chromosomes ranged from 50 to 56. On examination of the ultrastructure, membrane-bound dense-core neurosecretory-type granules were observed in the cytoplasm. Neuron-specific enolase (NSE) was immunocytochemically positive in the cytoplasm, and 9.3 ng/ml of NSE was detected in the cell culture supernatant. Human papillomavirus was not detected. In the p53 gene, a 3-bp deletion, AAC (Asn), was detected at codon 131 in exon 5. SKS exhibited good tumorigenicity, and the tumor doubling time was 11 days. Intraperitoneal injection of the cells caused peritoneal dissemination, and marked ascites formation was observed. SKS was highly sensitive to gemcitabine, and the 50% growth inhibitory concentration was 30 nM. SKS cells are useful as a model of neuroendocrine small cell carcinoma of the cervix, and chemotherapy using gemcitabine may possibly be effective in this malignancy. Copyright 2001 S. Karger AG, Basel.
机译：由患有宫颈神经内分泌小细胞癌的患者腹水建立的新细胞系（SKS）具有良好的致瘤性，并引起明显的腹膜扩散，并且在体外化学敏感性试验中对吉西他滨高度敏感。 SKS细胞是具有高核/细胞质（N / C）比的小圆形细胞，并长成集落状聚集体，形成漂浮细胞的球形聚集体。人口倍增时间为44小时。染色体数从50到56。检查超微结构后，在细胞质中观察到了膜结合的致密神经分泌型颗粒。神经元特异性烯醇化酶（NSE）在细胞质中呈免疫细胞化学阳性，在细胞培养上清液中检测到9.3 ng / ml NSE。未检测到人乳头瘤病毒。在p53基因中，在外显子5的第131位密码子处检测到3bp的缺失，AAC（Asn）。SKS显示出良好的致瘤性，并且肿瘤加倍时间为11天。腹膜内注射细胞引起腹膜扩散，并观察到明显的腹水形成。 SKS对吉西他滨高度敏感，其50％生长抑制浓度为30 nM。 SKS细胞可用作子宫颈神经内分泌小细胞癌的模型，使用吉西他滨的化学疗法可能对这种恶性肿瘤有效。版权所有2001 S. Karger AG，巴塞尔。

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