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首页> 外文期刊>Reproductive toxicology >Fertility of rabbit sperm exposed in vitro to cadmium and lead.
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Fertility of rabbit sperm exposed in vitro to cadmium and lead.

机译：体外暴露于镉和铅的兔精子的生育能力。

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摘要

The heavy metals Cd2+ and Pb2+ have been associated with male reproductive toxicology, including possible inhibition of sperm undergoing hyperactivated motility, indicative of capacitation. The objective of the present study was to test fertility of rabbit sperm exposed to Cd2+ or Pb2+ in vitro, followed by insemination of superovulated does. Semen was washed to remove seminal plasma and minimize possible binding of the heavy metals by proteins. Only 400,000 treated or control sperm were inseminated as a sensitive test of treatment, and the time sperm resided in the female before possible fertilization was varied by inseminating from 0 to 12 h after ovulating the does. Only 6 of 22 males tested showed appreciable spontaneous hyperactivation and neither Cd2+ nor Pb2+ affected hyperactivation, or presumably associated capacitation. Sperm from four of these six males were used to inseminate 66 rabbits, and 1483 oocytes and embryos were collected about 27 h later. No effect of 0.1 mM Cd2+ on fertilizing ability of sperm was found (75% fertilization with control sperm and 78% with treated sperm). With 0.025 mM Pb2+ the fertilization rate in pregnant does only was 82% for controls and lower (68%) with treated sperm. These tested concentrations of Cd2+ and Pb2+ are much higher than reported concentrations in semen of exposed workers.
机译：重金属Cd2 +和Pb2 +与男性生殖毒理学有关，包括可能抑制精子经历过度活化的运动，这表明获能。本研究的目的是在体外测试暴露于Cd2 +或Pb2 +的兔子精子的受精能力，然后进行超排卵的授精。清洗精液以去除精浆，并最大程度地减少蛋白质与重金属的结合。仅对受精或对照的受精精子进行授精，受精量为40万，在排卵后从0到12 h进行授精，从而改变了受精前女性在精子中的停留时间。在测试的22位男性中，只有6位表现出明显的自发性过度激活，而Cd2 +和Pb2 +均不影响过度激活或推测相关的获能。从这六只雄性中的四只精子中授精了66只兔子，大约27小时后收集了1483个卵母细胞和胚胎。未发现0.1 mM Cd2 +对精子受精能力的影响（对照精子受精率为75％，处理过的精子为78％）。使用0.025 mM Pb2 +时，对照组的孕妇受精率仅为82％，治疗精子的受精率较低（68％）。这些测试的Cd2 +和Pb2 +浓度远高于所报告的裸露工人精液中的浓度。

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