Halowax 1051 affects steroidogenesis, 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and cytochrome P450arom (CYP19) activity, and protein expression in porcine ovarian follicles.

Gregoraszczuk EL; Jerzak J; Rak Mardyla A; Falandysz J

首页> 外文期刊>Reproductive toxicology >Halowax 1051 affects steroidogenesis, 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and cytochrome P450arom (CYP19) activity, and protein expression in porcine ovarian follicles.

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Halowax 1051 affects steroidogenesis, 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and cytochrome P450arom (CYP19) activity, and protein expression in porcine ovarian follicles.

机译：Halowax 1051影响类固醇生成，17β-羟基类固醇脱氢酶（17beta-HSD）和细胞色素P450arom（CYP19）活性以及猪卵巢卵泡中的蛋白质表达。

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We evaluated the effect of Halowax 1051 on ovarian follicular testosterone (T) and estradiol (E2) secretion determined by EIA and on the expression of 17beta-HSD and CYP19 analyzed by Western blot. 17beta-HSD and CYP19 activity were determined indirectly by measuring the conversion of androstenedione (A4) to T and T to E2, respectively. Additionally, CYP19 activity by dibenzylfluorescein assay. Follicles were exposed to 1-1000pg/ml of Halowax 1051 for 24, 48 or 72h. It was found that Halowax 1051, in all doses used, increased basal T secretion concomitant with a decrease in basal E2 secretion. Inhibitory action on 17beta-HSD and stimulatory action on CYP19 (activity and protein expression) under the influence of 1pg/ml, and opposite effect under the influence of 10-1000pg/ml was noted. In conclusion, we suggest non-linear dose-response effect of Halowax 1051 on steroidogenesis and steroidogenic enzymes activity and protein expression.

机译：我们评估了Halowax 1051对通过EIA确定的卵巢滤泡性睾丸激素（T）和雌二醇（E2）分泌的影响，以及对通过蛋白质印迹分析的17beta-HSD和CYP19的表达的影响。通过分别测量雄烯二酮（A4）到T和T到E2的转化率间接确定17beta-HSD和CYP19活性。另外，通过二苄基荧光素测定法测定CYP19活性。卵泡暴露于1-1000pg / ml的Halowax 1051，持续24、48或72h。已发现，在所有剂量下，Halowax 1051的基础T分泌增加，而基础E2分泌减少。注意到在1pg / ml的影响下对17beta-HSD的抑制作用和对CYP19的刺激作用（活性和蛋白质表达），在10-1000pg / ml的影响下表现出相反的作用。总之，我们建议Halowax 1051对类固醇生成，类固醇生成酶活性和蛋白质表达具有非线性剂量效应。

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《Reproductive toxicology》 |2011年第4期|共6页
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Gregoraszczuk EL; Jerzak J; Rak Mardyla A; Falandysz J;
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中图分类毒物学（毒理学）;
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1. Halowax 1051 affects steroidogenesis, 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and cytochrome P450arom (CYP19) activity, and protein expression in porcine ovarian follicles. [J] . Gregoraszczuk EL, Jerzak J, Rak Mardyla A, Reproductive toxicology . 2011,第4期

机译：Halowax 1051影响类固醇生成，17β-羟基类固醇脱氢酶（17beta-HSD）和细胞色素P450arom（CYP19）活性以及猪卵巢卵泡中的蛋白质表达。
2. Effects of individual polychlorinated naphthalene (PCN) components of Halowax 1051 and two defined, artificial PCN mixtures on AHR and CYP1A1 protein expression, steroid secretion and expression of enzymes involved in steroidogenesis (CYP17, 17 beta-HSD and CYP19) in porcine ovarian follicles [J] . Barc Justyna, Gregoraszczuk Ewa Lucja Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems . 2014,第Null期

机译：Halowax 1051的各个多氯萘（PCN）组分和两种确定的人工PCN混合物对猪卵巢卵泡中AHR和CYP1A1蛋白表达，类固醇分泌和涉及类固醇生成的酶（CYP17、17 beta-HSD和CYP19）表达的影响
3. Congener-specific action of PBDEs on steroid secretion, CYP17, 17beta-HSD and CYP19 activity and protein expression in porcine ovarian follicles. [J] . Karpeta A, Rak Mardyla A, Jerzak J, Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research . 2011,第3期

机译：PBDEs对猪卵泡中类固醇分泌，CYP17、17beta-HSD和CYP19活性及蛋白表达的同类物特异性作用。
4. Urinary Bisphenol A Concentrations and Cytochrome P450 19 A1 (Cyp19) Gene Expression in Ovarian Granulosa Cells: An in vivo Human Study [O] . Shelley Ehrlich, Paige L. Williams, Russ Hauser, -1

机译：卵巢双颗粒A浓度和细胞色素P450 19 A1（Cyp19）基因在卵巢颗粒细胞中的表达：体内人类研究。
5. Participation of Mitogen-activated Protein Kinase in Luteinizing Hormone-induced Differential Regulation of Steroidogenesis and Steroidogenic Gene Expression in Mural and Cumulus Granulosa Cells of Mouse Preovulatory Follicles. [O] . Su, You-Qiang, Nyegaard, Mette, Overgaard, Michael Toft, 2006

机译：丝裂素活化蛋白激酶参与促黄体激素诱导的小鼠排卵前卵泡壁细胞和卵泡颗粒细胞中类固醇生成和类固醇基因表达的差异调节。

Halowax 1051 affects steroidogenesis, 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and cytochrome P450arom (CYP19) activity, and protein expression in porcine ovarian follicles.

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