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首页> 外文期刊>Reproductive toxicology >Genome-wide DNA methylation patterns in IVF-conceived mice and their progeny: a putative model for ART-conceived humans.
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Genome-wide DNA methylation patterns in IVF-conceived mice and their progeny: a putative model for ART-conceived humans.

机译：IVF受孕小鼠及其后代的全基因组DNA甲基化模式：ART受孕人类的推定模型。

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摘要

The aim of this study was to use a mouse model to gain an understanding of the safety of reproduction between humans conceived through assisted reproductive technology (ART). Mice derived from in vitro fertilization and embryo transfer (IVF-ET) were crossed. Their behavior, morphology, histology and genome-wide DNA methylation status in the brain were examined by the Morris water maze, H&E staining and methylated DNA immunoprecipitation coupled with DNA methylation microarrays. Although no significant differences in behavior or morphology were observed, we did find small clusters of CpG islands and promoters that were aberrantly methylated. Hypermethylation was more common than hypomethylation in each of the two generations. Some of the aberrant methylated promoters were validated by bisulfite sequencing. Our results show that IVF may slightly modify the somatic methylation pattern and that some of this aberrant methylation might be inherited by the following generation.
机译：这项研究的目的是使用小鼠模型来了解通过辅助生殖技术（ART）构想的人类之间生殖的安全性。杂交了来自体外受精和胚胎移植（IVF-ET）的小鼠。通过莫里斯水迷宫，H＆E染色和甲基化DNA免疫沉淀结合DNA甲基化微阵列检查了它们在大脑中的行为，形态，组织学和全基因组DNA甲基化状态。尽管没有观察到行为或形态上的显着差异，但我们确实发现了小簇的CpG岛和异常甲基化的启动子。在两代中的每一代中，高甲基化比低甲基化更为普遍。通过亚硫酸氢盐测序验证了一些异常的甲基化启动子。我们的结果表明，IVF可能会稍微改变体细胞甲基化模式，并且某些异常的甲基化可能会在后代遗传。

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