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首页> 外文期刊>Reproductive toxicology >The mammalian testis accumulates lower levels of organochlorine chemicals compared with other tissues.
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The mammalian testis accumulates lower levels of organochlorine chemicals compared with other tissues.

机译：与其他组织相比，哺乳动物的睾丸中积累的有机氯化学物质含量较低。

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Tissues were obtained from three separate experiments in order to quantify the tissue distribution of organochlorine chemicals that are thought to be potential reproductive toxicants in males: 1) Sprague Dawley rats received 1 microCi of 14C-Aldrin or 14C-Dieldrin (20.6 microCi/micromole) i.p. once a week for three weeks. One week and four weeks after the last injection, tissues were harvested and stored at -80 degrees C. Tissue 14C levels were quantified by scintillation spectrometry. 2) Cis- or trans-nonachlor (0, 0.25, 2.5, 25 mg/kg body weight) were administered daily in corn oil to male rats by gavage for 28 days. Tissues were harvested and frozen at -80 degrees C on the 29th day. Organochlorine residues were extracted and quantified by gas chromatography with electron capture detection. 3) Technical grade toxaphene (0, 0.1, 0.4 or 0.8 mg/kg body weight) was ingested daily by female cynomolgus monkeys of reproductive age for 18 months prior to being mated with control males. Dosing continued during pregnancy and lactation. Their infants received toxaphene via breast milk, and upon weaning, they ingested the same dose as their mothers for 48 to 49 weeks until, at 77 to 80 weeks of age, tissues were harvested and stored at -80 degrees C. Organochlorine residues were extracted and quantified as previously stated. In all three experiments, organochlorine residues in the testis were lower than in most of the other reproductive tract and nonreproductive tract tissues we examined. For example, testicular aldrin and dieldrin levels were <5% the epididymal content; testicular cis- and trans-nonachlor were <25% the epididymal content and, testicular toxaphene levels were <15% of the epididymal content. The reasons for the low degree of accumulation by the testis in comparison with other tissues are unknown. However, the lower testicular content may afford germ cells some protection from the potentially toxic effects of these chemicals.
机译：从三个独立的实验中获得了组织，以便量化有机氯化学物质在男性中的潜在生殖毒性物质的组织分布：1）Sprague Dawley大鼠接受了1 microCi的14C-Aldrin或14C-Dieldrin（20.6 microCi / micromole） ip每周一次，持续三个星期。最后一次注射后一个星期和四个星期，收获组织并将​​其储存在-80℃。通过闪烁光谱法定量组织14C水平。 2）每天在玉米油中通过管饲法向雄性大鼠施用顺-或反-壬草胺（0、0.25、2.5、25mg / kg体重），持续28天。在第29天收获组织并在-80℃下冷冻。提取有机氯残留物，并通过带电子捕获检测的气相色谱法进行定量。 3）在与对照雄性交配之前，育龄雌性食蟹猴每天摄入工业级毒杀芬（0、0.1、0.4或0.8 mg / kg体重）。在怀孕和哺乳期间继续给药。他们的婴儿通过母乳接受毒杀芬，断奶后，他们以与母亲相同的剂量摄入了48至49周，直到77至80周龄，收集了组织并保存在-80摄氏度。提取了有机氯残留物并如前所述量化。在所有三个实验中，睾丸中的有机氯残留量均低于我们检查的大多数其他生殖道和非生殖道组织。例如，睾丸艾氏剂和狄氏剂的含量低于附睾含量的5％。睾丸中顺式和反式壬草胺的含量低于附睾含量的25％，睾丸中的杀虫酚水平低于附睾含量的15％。与其他组织相比，睾丸积聚程度低的原因尚不清楚。然而，较低的睾丸含量可以使生殖细胞免受这些化学物质的潜在毒性影响。

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