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首页> 外文期刊>Reproductive toxicology >The effect of methoxychlor on the epididymal antioxidant system of adult rats.
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The effect of methoxychlor on the epididymal antioxidant system of adult rats.

机译：甲氧氯对成年大鼠附睾抗氧化系统的影响。

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Methoxychlor is widely used as a pesticide in many countries and has been shown to induce reproductive abnormalities in male rats, causing reduced fertility. The mechanism of action of methoxychlor on the male reproductive system is not clear. In the present study we investigated whether administration of methoxychlor induces oxidative stress in the epididymis and epididymal sperm of adult rats. Methoxychlor (50, 100, or 200 mg/kg body weight/day) was administered orally for 1, 4, or 7 days. The animals were killed using anesthetic ether 24 h after of the last treatment. Epididymal sperm were collected by cutting the epididymis into small pieces in Ham's F-12 medium at 35 degrees C. The body weight and weights of the testis, liver, and kidney did not show any significant changes in the methoxychlor-treated rats. The weight of the epididymis, seminal vesicles, and ventral prostate as well as epididymal sperm counts decreased after 50, 100, or 200 mg/kg/day for 7 days but remained unchanged after shorter courses of treatment. Epididymal sperm motility was decreased in a dose-dependent manner in the animals treated with methoxychlor for 4 or 7 days. The activities of the antioxidant enzymes superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase were decreased while the levels of hydrogen peroxide and lipid peroxidation were increased in the epididymal sperm as well as in the caput, corpus, and cauda epididymis after 4 or 7 days of treatment. The activities of superoxide dismutase decreased while the levels of lipid peroxidation increased in the liver but not in the kidney in all groups. Co-administration of the antioxidant vitamin E (20 mg/kg body weight/ day) to the 200 mg/kg/d methoxychlor-treated rats for 7 days prevented significant changes in the antioxidant systems in the epididymis and epididymal sperm and prevented alterations in sperm counts and motility. The results indicated that methoxychlor induces oxidative stress in the epididymis and epididymal sperm by decreasing antioxidant enzymes, possibly by inducing reactive oxygen species. In conclusion the adverse effect of methoxychlor on the male reproduction could be due to induction of oxidative stress.
机译：甲氧氯在许多国家被广泛用作杀虫剂，并已证明可引起雄性大鼠生殖异常，从而导致生育力下降。甲氧基氯对雄性生殖系统的作用机理尚不清楚。在本研究中，我们调查了甲氧氯的施用是否在成年大鼠的附睾和附睾精子中诱导氧化应激。口服甲氧氯（50、100或200毫克/千克体重/天），持续1、4或7天。在最后一次治疗后24小时，用麻醉乙醚杀死动物。通过在35摄氏度的Ham's F-12培养基中将附睾切成小块来收集附睾的精子。在甲氧氯处理的大鼠中，体重和睾丸，肝脏和肾脏的体重没有显示任何明显变化。附睾，精囊和腹侧前列腺的重量以及附睾的精子数量在每天50、100或200 mg / kg连续7天后下降，但在较短的疗程后仍保持不变。在用甲氧氯处理4或7天的动物中，附睾精子运动以剂量依赖性方式降低。 4或7天后，附睾精子，以及附睾，cap体和马尾附睾中抗氧化酶超氧化物歧化酶，过氧化氢酶，谷胱甘肽还原酶和谷胱甘肽过氧化物酶的活性降低，而过氧化氢和脂质过氧化的水平升高。天的治疗。在所有组中，肝脏中超氧化物歧化酶的活性降低，而脂质过氧化水平升高，而肾脏中没有。将抗氧化剂维生素E（20 mg / kg体重/天）与200 mg / kg / d甲氧基氯处理的大鼠共同给药7天，可防止附睾和附睾精子中抗氧化剂系统的显着变化，并防止其改变。精子数量和活力。结果表明，甲氧氯可通过减少抗氧化酶，可能通过诱导活性氧来诱导附睾和附睾精子的氧化应激。总之，甲氧基氯对雄性生殖的不利影响可能是由于氧化应激的诱导。

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