...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Reproductive toxicology >Maternal exposure to environmental tobacco smoke, GSTM1/T1 polymorphisms and oxidative stress.
【24h】

Maternal exposure to environmental tobacco smoke, GSTM1/T1 polymorphisms and oxidative stress.

机译：孕妇暴露于环境烟草烟雾，GSTM1 / T1多态性和氧化应激。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Environmental tobacco smoking (ETS) is known to be associated with adverse pregnancy outcomes. The purpose of this study was to investigate the relationship between maternal exposure to ETS and oxidative stress for neonates, as well as the effect of maternal genetic polymorphisms, glutathione-S-transferase M1 (GSTM1) and GSTT1, on this relationship. We used the radioimmunoassay to measure the urinary concentration of cotinine in 266 pregnant women who denied smoking cigarettes during pregnancy and in their singleton babies. In addition, the urinary concentration of malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OH-dG) were assessed using high-performance liquid chromatography and enzyme-linked immunosorbent assay, respectively. We also extracted DNA from whole blood obtained from the mothers and then conducted polymerase chain reaction on the samples to determine the GSTM1 and GSTT1 genotypes. The maternal cotinine concentration was found to be significantly associated with the fetal cotinine concentration, particularly for mothers whose urine cotinine concentrations were above 120 microg/gcr (p<0.01). The fetal urine cotinine concentration was also found to be significantly associated with the fetal urine MDA concentration (p<0.01). When the null type maternal GSTM1 or the wild type GSTT1 was present, the maternal oxidative stress level increased significantly as the maternal continine concentration increased (MDA: p<0.01; 8-OH-dG: p<0.01). No significant relationships were found between maternal cotinine and fetal oxidative stress markers, however, the fetal MDA levels increased significantly as fetal cotinine levels increased. These results suggest that the maternal exposure to ETS affects the fetal urine cotinine concentration and induces production of maternal oxidative stress. In addition, maternal genetic polymorphisms of GSTM1 and GSTT1 may modify the oxidative stress by maternal exposure to ETS.
机译：已知环境吸烟（ETS）与不良妊娠结局有关。这项研究的目的是调查母体暴露于ETS与新生儿氧化应激之间的关系，以及母体遗传多态性，谷胱甘肽S-转移酶M1（GSTM1）和GSTT1的影响。我们使用放射免疫测定法测量了266名在怀孕期间拒绝吸烟的孕妇及其单身婴儿的尿中可替宁的浓度。此外，分别使用高效液相色谱法和酶联免疫吸附法评估了丙二醛（MDA）和8-羟基-2-脱氧鸟苷（8-OH-dG）的尿液浓度。我们还从母亲那里获得的全血中提取了DNA，然后对样品进行了聚合酶链反应，以确定GSTM1和GSTT1基因型。发现母亲可替宁浓度与胎儿可替宁浓度显着相关，特别是对于尿可替宁浓度高于120 microg / gcr的母亲（p <0.01）。还发现胎儿尿中的可替宁浓度与胎儿尿中的MDA浓度显着相关（p <0.01）。当存在无效型母体GSTM1或野生型GSTT1时，随着母体尿素浓度的增加，母体氧化应激水平显着增加（MDA：p <0.01; 8-OH-dG：p <0.01）。孕妇可替宁与胎儿氧化应激指标之间未发现显着相关性，但是，随着胎儿可替宁水平的升高，胎儿的MDA水平也显着增加。这些结果表明，母体接触ETS会影响胎儿尿中可替宁的浓度，并诱导母体氧化应激的产生。此外，孕妇的GSTM1和GSTT1遗传多态性可能通过孕妇暴露于ETS来改变氧化应激。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号