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首页> 外文期刊>Reproductive toxicology >Male rats exposed in utero to di(n-butyl) phthalate: Age-related changes in Leydig cell smooth endoplasmic reticulum and testicular testosterone-biosynthesis enzymes/proteins
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Male rats exposed in utero to di(n-butyl) phthalate: Age-related changes in Leydig cell smooth endoplasmic reticulum and testicular testosterone-biosynthesis enzymes/proteins

机译：子宫内暴露于邻苯二甲酸二正丁酯的雄性大鼠：Leydig细胞平滑内质网和睾丸睾丸激素生物合成酶/蛋白质的年龄相关变化

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摘要

This study investigated the age-related (i.e., weeks 5, 7, 9,14 and 17) morphological changes of Leydig cell smooth endoplasmic reticulum (LCs-ER) and testicular testosterone biosynthesis/protein expression in rats in utero exposed to di(n-butyl) phthalate (DBP) (intragastrically; 100 mg/kg/day) on days 12-21 post conception. Ultrastructural observations revealed the LCs-ER of the DBP group were non-dilated until peri-puberty, and thereafter decreased and disappeared. RT-PCR and Western blotting analyses revealed that StAR and P450scc levels in the DBP group were significantly lower at 5 and 7 weeks compared with the vehicle group but became similar during weeks 9-17. Although 3 beta-HSD, P450c17, and 17 beta-FISD levels of mRNA and protein in the DBP group were similar to the vehicle control group at 5 and 7 weeks of age, they were significantly lower during weeks 9-17. In utero DBP exposure results in age-related LCs-ER changes corresponding to reduction of testicular testosterone biosynthesis enzymes/associated proteins. (C) 2016 Published by Elsevier Inc.
机译：本研究调查了暴露于di（n）的子宫内大鼠的Leydig细胞平滑内质网（LCs-ER）和睾丸睾丸激素的合成/蛋白质表达与年龄相关的（即第5、7、9、14和17周）形态变化。怀孕后第12-21天的邻苯二甲酸丁酯（DBP）（胃内; 100 mg / kg /天）。超微结构观察显示，DBP组的LCs-ER直到青春期前都没有扩张，然后下降并消失。 RT-PCR和蛋白质印迹分析显示，DBP组在第5周和第7周时的StAR和P450scc水平与媒介物组相比明显降低，但在9-17周时变得相似。尽管DBP组中3个beta-HSD，P450c17和17个beta-FISD的mRNA和蛋白质水平在5和7周龄时与媒介物对照组相似，但在9-17周时显着降低。在子宫内，DBP暴露导致与年龄有关的LC-ER变化，这对应于睾丸睾丸激素生物合成酶/相关蛋白的减少。 （C）2016由Elsevier Inc.发布

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