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首页> 外文期刊>Russian Journal of Plant Physiology >Effect of toxic Fe2+ levels on the biological characteristics of rice root border cells.
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Effect of toxic Fe2+ levels on the biological characteristics of rice root border cells.

机译：有毒的Fe 2 + 水平对水稻根缘细胞生物学特性的影响。

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Root border cells are a population of rhizosphere cells surrounding the root tips but separated from them. The root tip is a major target of Fe2+ toxicity; thus, it was hypothesized that the border cells might protect or exacerbate Fe2+ toxicity. To explore the effects of excess Fe2+ on the border cells in rice (Oryza sativa L.), experiments were carried out using the border cells in vitro (Shanyou No. 10). The border cells were precultured under "hanging in the air" and detached from the root tips. The shape, numbers, and viability of border cells were examined during exposure to toxic levels of Fe2+. When the root was 1 mm long, there were 205 border cells on average. With the growth of the root, more border cells were observed. When the root grew to 25 mm long, the total number of border cells reached a maximum, while the maximum activity of border cells appeared when the root was 20 mm long. The pectin methyl esterase (PME) activity of the root cap peaked at a root length of 2 mm. Border cell development was related to PME activity in rice. Excessive Fe2+ was toxic to detached border cells. After treatment with 200 micro M Fe2+ solution for 48 h, cell viability decreased by 72.70%. However, when treated with 400 micro M Fe2+ solution, the number of viable cells was actually higher, suggesting the induction of a cellular self-protection response. The activity of PME first increased under high concentrations of Fe2+ and then decreased. These results indicate that toxic levels of Fe2+ modulate PME activity and border cell survival.
机译：根边界细胞是根尖周围但与根尖分开的根际细胞群体。根尖是Fe 2 + 毒性的主要目标。因此，人们推测边界细胞可能保护或加剧Fe 2 + 的毒性。为了研究过量的Fe 2 + 对水稻（Oryza sativa L.）边缘细胞的影响，利用该边缘细胞在体外进行了实验（汕优10号）。边缘细胞在“悬于空中”下进行预培养，并与根尖分离。在暴露于毒性水平的Fe 2 + 期间检查了边缘细胞的形状，数量和活力。当根长1毫米时，平均有205个边界细胞。随着根的生长，观察到更多的边界细胞。当根长到25 mm长时，边界细胞的总数达到最大值，而当根长20 mm时，边界细胞的最大活性出现。根冠的果胶甲基酯酶（PME）活性在2 mm的根长处达到峰值。边界细胞发育与水稻中的PME活性有关。 Fe 2 + 过量对分离的边缘细胞有毒。 200μMFe 2 + 溶液处理48 h后，细胞活力降低了72.70％。然而，当用400μM的Fe 2 + 溶液处理时，活细胞的数量实际上更高，表明诱导了细胞自我保护反应。在高浓度的Fe 2 + 下，PME的活性先升高，然后降低。这些结果表明，Fe 2 + 的毒性水平调节PME活性和边界细胞存活。

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