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首页> 外文期刊>Russian Journal of Plant Physiology >Abscisic acid enhanced ajmalicine biosynthesis in hairy roots of Rauvolfia verticillata by upregulating expression of the MEP pathway genes.
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Abscisic acid enhanced ajmalicine biosynthesis in hairy roots of Rauvolfia verticillata by upregulating expression of the MEP pathway genes.

机译：脱落酸可通过上调MEP途径基因的表达来增强轮状红毛线菌毛状根中的茉莉花生物合成。

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摘要

Hairy root cultures of Rauvolfia verticillata were established and used to analyze the expression of the eight ajmalicine-pathway genes (the five MEP pathway genes, including DXR, MCT, MECS, HDS, and HDR}; the three ajmalicine-pathway-specific genes, including TDC, STR, and SGD) and ajmalicine accumulation in the cultures treated with ABA. In the ABA treatment, the five MEP-pathway genes were simultaneously up-regulated at the transcriptional level, while the three ajmalicine-pathway-specific genes, including TDC, STR, and SGD were not up-regulated. The statistical analysis showed that the MEP pathway gene expression levels in the ABA treatment were significantly different from those in the control. TDC showed no response to ABA treatment, and SGD expression decreased slightly. Surprisingly, the well-known rate-limiting enzyme gene STR showed dramatically decreased expression in the ABA treatment. The HPLC analysis showed that the content of ajmalicine in the ABA-treated hairy roots of R. verticillata (196.9+or-3.6 micro g/g dry wt) was significantly higher than that in the control (171.0+or-7.6 micro g/g dry wt). This suggests that the higher expression levels of the MEP pathway genes could enhance ajmalicine biosynthesis and the MEP pathway plays an important role in ajmalicine biosynthesis in R. verticillata. Finally, STR might not be the rate-limiting enzyme involved in ajmalicine biosynthesis of R. verticillata.
机译：建立了黄萎病菌的毛状根培养物，并将其用于分析八种茉莉花碱途径基因（五种MEP途径基因，包括DXR，MCT，MECS，HDS和HDR}的表达;三种茉莉花碱途径特异性基因，包括TDC，STR和SGD）和阿马利碱在ABA处理的培养物中的积累。在ABA处理中，五个MEP途径基因在转录水平上同时被上调，而三个杜鹃花途径特异性基因（包括TDC，STR和SGD）未被上调。统计分析表明，ABA处理中的MEP途径基因表达水平与对照组相比有显着差异。 TDC对ABA处理无反应，SGD表达略有下降。出人意料的是，众所周知的限速酶基因STR在ABA处理中显示出明显降低的表达。 HPLC分析表明，ABA处理的轮枝红毛菜根中的杜鹃花含量（196.9 +或-3.6 micro g / g干重）显着高于对照（171.0 +或-7.6 micro g / g）。克干重）。这表明，MEP途径基因的较高表达水平可以增强阿美利辛的生物合成，并且MEP途径在黄萎病菌的阿美利辛的生物合成中起重要作用。最后，STR可能不是黄萎病菌阿马利菊酯生物合成中的限速酶。

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