• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Signalling >Expression of plasma membrane calcium pump isoform mRNAs in breast cancer cell lines
【24h】

Expression of plasma membrane calcium pump isoform mRNAs in breast cancer cell lines

机译：浆膜钙泵同工型mRNA在乳腺癌细胞株中的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The plasma membrane Ca2+ ATPase (PMCA) is an important regulator of free intracellular calcium, with dynamic regulation in the rat mammary gland during lactation. Recent studies suggest that Ca2+ plans a role in cellular proliferation, To determine if PMCA expression is altered in tumorigenesis. Ne compared relative levels of PMCA1 mRNA. We found that the relative expression of PMCA1 mRNA is increased, by approximately 270% and 170%, in MCF-7 and MDA-MB-231 human breast cancer cell lines deprived of serum for 72 h, respectively, compared to the similarly treated MCF-10A human mammary, gland epithelial cell line. Characterization of PMCA mRNA isoforms revealed that PMCA1b and PMCA4 mRNA are expressed in MCF-7, MDA-MB-231. SK-BR-3, ZR-75-1 and BT-483 breast cancer cell lines, We also detected PMCA2 mRNA expression in all the breast cancer cell lines examined. However, PMCA, mRNA was only detected in BT-483 cells, Our results suggest that PMCA expression may be altered in breast cancer cell lined, suggesting altered Ca2+ regulation in these cell lines. Our results also indicate that breast cancer cell lines can express mRNAs for a variety PMCA isoforms. (C) 2002 Elsevier Science Inc. All rights reserved. [References: 45]
机译：质膜Ca2 + ATPase（PMCA）是游离细胞内钙的重要调节剂，在哺乳期大鼠乳腺中具有动态调节作用。最近的研究表明Ca2 +计划在细胞增殖中起作用，以确定在肿瘤发生过程中PMCA表达是否改变。 Ne比较了PMCA1 mRNA的相对水平。我们发现，与类似处理的MCF相比，在无血清的MCF-7和MDA-MB-231人乳腺癌细胞系中，PMCA1 mRNA的相对表达分别增加了约270％和170％。 -10A人乳腺，腺上皮细胞系。 PMCA mRNA同工型的表征显示PMCA1b和PMCA4 mRNA在MCF-7，MDA-MB-231中表达。 SK-BR-3，ZR-75-1和BT-483乳腺癌细胞系，我们还在检测的所有乳腺癌细胞系中检测到PMCA2 mRNA表达。然而，仅在BT-483细胞中检测到PMCA，mRNA。我们的结果表明，在乳腺癌细胞系中PMCA的表达可能发生了改变，这提示这些细胞系中Ca2 +调控的改变。我们的结果还表明，乳腺癌细胞系可以表达多种PMCA亚型的mRNA。 （C）2002 Elsevier Science Inc.保留所有权利。 [参考：45]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号