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首页> 外文期刊>Ophthalmic genetics >Expression of double strand DNA breaks repair genes in pterygium.
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Expression of double strand DNA breaks repair genes in pterygium.

机译：双链DNA的表达破坏翼状repair肉中的修复基因。

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PURPOSE: The alteration in the expression of some genes and proteins responsible for chosen DNA repair pathways in pterygium pathogenesis were studied. This study was focused on the examination of the expression of genes and RAD50 protein taking part in homologous recombination. METHODS: Peripheral blood lymphocytes, samples of pterygium tissue, samples of conjunctiva of patients suffering from pterygium as well as peripheral blood lymphocytes and conjunctiva of patients from the control group were examined. In order to identify genes products from RNA, Ribonuclease Protection Assai method was applied. LIM15, RAD50, RAD54, RAD52, MRE11, XRCC2, XRCC3, RAD51, RAD51B, RAD51C, RAD51D genes transcripts were detected. Expression of RAD50 protein was analyzed immunohistochemically. RESULTS: Peripheral blood lymphocytes analyses revealed lower level of RAD50 gene expression in the pterygium patients compared to the control group and the increased expression of XRCC2, XRCC3 and RAD51 genes in patients with pterygium, who declared the recurrence of the lesion in comparison to the patients with primary pterygium. Lower expression of the RAD54 gene in pterygium tissue comparing to conjunctiva from the eyes with pterygium was found. An expression of RAD50 gene in the conjunctiva originating from eyes with pterygium in comparison to the conjunctiva of control group was shown to be considerably higher. Expression of RAD50 protein in pterygium squamous epithelial cells was significantly higher than in conjunctiva from control group. CONCLUSION: There may exist a relationship between pterygium pathogenesis and damages of double strand DNA, however, the elucidation of its exact nature needs further study.
机译：目的：研究翼状path肉发病机理中某些基因和蛋白质的表达变化，这些基因和蛋白质负责选择的DNA修复途径。这项研究集中在基因表达和RAD50蛋白参与同源重组的检查。方法：检查对照组外周血淋巴细胞，翼状tissue肉组织样本，翼状patients肉患者的结膜样本以及外周血淋巴细胞和结膜。为了鉴定RNA的基因产物，应用了核糖核酸酶保护Assai方法。检测到LIM15，RAD50，RAD54，RAD52，MRE11，XRCC2，XRCC3，RAD51，RAD51B，RAD51C，RAD51D基因转录本。免疫组化分析RAD50蛋白的表达。结果：外周血淋巴细胞分析显示，翼状patients肉患者的RAD50基因表达水平低于对照组，翼状X肉患者的XRCC2，XRCC3和RAD51基因表达水平也有所提高，与那些患者相比，翼状ery肉患者复发与原发性翼状。肉。发现与翼状eyes肉眼结膜相比，翼状tissue肉组织中RAD54基因的表达较低。与对照组的结膜相比，RAD50基因在翼状pt肉的眼结膜中的表达明显更高。翼状肉鳞状上皮细胞中RAD50蛋白的表达显着高于对照组结膜。结论：翼状ery肉的发病机制与双链DNA的损伤可能存在一定的关系，但其确切性质的阐明尚需进一步研究。

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